Immunodotting assay

Manufactured by EUROIMMUN

Sourced in Germany

The Immunodotting assay is a diagnostic tool used in clinical laboratories. It is designed to detect and identify specific antibodies or antigens in a sample. The assay involves the application of a sample onto a membrane or strip, followed by the detection of target analytes through the use of labeled reagents. The core function of the Immunodotting assay is to provide a qualitative or semi-quantitative analysis of the presence and/or amount of the target molecules in the tested sample.

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Lab products found in correlation

Indirect immunofluorescence assay, by EUROIMMUN (5 mentions) Elisa, by EUROIMMUN (3 mentions) Crithidia luciliae indirect immunofluorescence test, by EUROIMMUN (2 mentions) Rate nephelometry assay, by Beckman Coulter (1 mentions) Immage, by Beckman Coulter (1 mentions)

5 protocols using immunodotting assay

Comprehensive Lipid and Autoantibody Profiling in Kidney Disease

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The levels of blood lipids, including triglyceride (TG) (BIOSINO BIO-TECHNOLOGY&SCIENCE INC, Beijing, China; normal range: 0.56–1.7mmol/L),total cholesterol (TCHO) (BIOSINO BIO-TECHNOLOGY&SCIENCE INC, Beijing, China; normal range: 3.4–5.2mmol/L), high-density lipoprotein cholesterol (HDL-C) (SEKISUI MEDICAL TECHNOLOGY (CHINA) LTD, Beijing, China; normal range: 0.9–1.4mmol/L) and low-density lipoprotein cholesterol (LDL-C) (SEKISUI MEDICAL TECHNOLOGY (CHINA) LTD, Beijing, China; normal range: 2.1–3.1mmol/L), for all the patients were detected before the day of renal biopsy.
Serum antinuclear antibodies (ANA) were detected using indirect immunofluorescence assay (EUROIMMUN, Lübeck, Germany) and anti-double-stranded DNA antibodies were detected using Crithidia luciliae indirect immunofluorescence test (EUROIMMUN, Lübeck, Germany). Anti-extractable nuclear antigen (ENA) antibodies, including anti-Sm, anti-SSA, anti-SSB and anti-RNP antibody, were detected using immunodotting assay (EUROIMMUN, Lübeck, Germany). Anti-cardiolipin antibodies were detected using ELISA (enzyme-linked immunosorbent assay) (EUROIMMUN). Serum C3 was determined using rate nephelometry assay (Beckman-Coulter, IMMAGE, USA, normal range>0.85g/L).

Huang J., Han S.S., Qin D.D., Wu L.H., Song Y., Yu F., Wang S.X., Liu G, & Zhao M.H. (2015). Renal Interstitial Arteriosclerotic Lesions in Lupus Nephritis Patients: A Cohort Study from China. PLoS ONE, 10(11), e0141547.

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Diagnostic Utility of Salivary Gland Biopsy

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ANA were detected using an indirect immunofluorescence assay (EUROIMMUN, Lübeck, Germany), and anti-extractable nuclear antigen antibodies, including anti-SSA, anti-Ro52, and anti-SSB/La antibodies, were measured using immunodotting assay (EUROIMMUN). In this study, most patients had positive ANA and ANA titers were recorded as four grades (1:100, 1:320, 1:1000, ≥ 1:3200). All patients underwent LSG biopsies. Sufficient LSGs require at least four minor salivary glands to ensure a minimum glandular surface area of 8 mm², but if the minor salivary glands are small, this number rises to six. The oral pathologist then has sufficient sample to determine the focus score or the presence of NSCS [4 (link)]. H&E sections were assessed by the same experienced pathologist, and pathology reports were recorded according to the Chisholm and Mason grading system (grades 0–4) [15 (link)]. FS was calculated for each patient.

Li H.X., Wang Y.F., Zhou Y.X., Feng Y, & Wu Z.B. (2022). Characteristics of Patients with Primary Sjögren’s Syndrome and Non-specific Chronic Sialadenitis: A Subtype in Elderly Patients. Rheumatology and Therapy, 9(5), 1347-1359.

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Evaluating Autoantibodies and Salivary Gland Pathology in pSS

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Anti-nuclear antibodies (ANA) were detected using an indirect immunofluorescence assay (EUROIMMUN, Lübeck, Germany), and anti-extractable nuclear antigen antibodies including anti-SSA, anti-SSB, and anti-Ro-52 antibodies were measured using immunodotting assay (EUROIMMUN, Lübeck, Germany). In this study, all pSS patients were ANA positive and ANA titres were graded at 4 levels (1:320, 1:1000, 1:3200, and 1:10000).
All patients underwent LSG biopsies. Hematoxylin and eosin (H&E) -stained sections were assessed by the same experienced pathologist, and pathology reports were recorded according to the Chisholm and Mason grading system (grade 0–4) [19 (link)]. The focus score (FS) which referred to the mean number of mononuclear cell infiltrates with 50 or more inflammatory cells per 4 mm² of periductal or perivascular tissue [20 (link)] was recorded for each patient.

Li H., Zhou Y., Wang P., Wang Y., Feng Y., Zhang Y, & Wu Z. (2023). Alterations of CD8+ T cells in the blood and salivary glands of patients with primary Sjögren’s syndrome. Clinical Rheumatology, 42(5), 1327-1338.

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Autoimmune Antibody Profiling in Renal Biopsy

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The following laboratory features were further detected using serum or plasma at the day of renal biopsy.
Serum antinuclear antibodies (ANA) were detected using indirect immunofluorescence assay (EUROIMMUN, Lübeck, Germany) and anti-double-stranded DNA (ds-DNA) antibodies were detected using Crithidia luciliae indirect immunofluorescence test (EUROIMMUN, Lübeck, Germany). Anti-extractable nuclear antigen (ENA) antibodies, including anti-Sm, anti-SSA, anti-SSB, and anti-RNP antibodies, were detected using immunodotting assay (EUROIMMUN, Lübeck, Germany). Anti-cardiolipin antibodies and anti-β₂GP-1 antibodies were detected using enzyme-linked immunosorbent assay (ELISA) (EUROIMMUN, Lübeck, Germany).

Li Q., Song D., Wang F., Tan Y., Yu F, & Zhao M. (2014). Clinicopathological Characteristics and Outcomes of Chinese Patients with Scanty Immune Deposits Lupus Nephritis: A Large Cohort Study from a Single Center. The Scientific World Journal, 2014, 212597.

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Autoantibody Detection in Clinical Samples

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Serum antinuclear antibodies (ANA) and anti-double-stranded DNA antibodies were detected using the indirect immunofluorescence assay (EUROIMMUN, Lübeck, Germany). Anti-cardiolipin antibodies were detected using enzyme-linked immunosorbent assay (ELISA) (EUROIMMUN). Anti-ENA antibodies were detected using an immunodotting assay (EUROIMMUN). Serum C3 was determined using the rate nephelometry assay (IMMAGE; Beckman-Coulter, Brea, CA; normal range >0.85 g/l).

Yuan M., Tan Y., Pang Y., Li Y.Z., Song Y., Yu F, & Zhao M.H. (2017). Anti-pentraxin 3 auto-antibodies might be protective in lupus nephritis: a large cohort study. Renal Failure, 39(1), 465-473.

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