Wound samples were received as swabs (described above) 1 day after sample were taken. A dilution series was made using sterile PBS (pH 7.2), and samples were inoculated onto tryptone soy broth agar (Oxoid, Basingstoke, Hampshire, England) plates supplemented with, respectively, horse blood and haematein for aerobic incubation and onto equal plates and fastidious anaerobe agar (FAA, Oxoid) plates, supplemented with horse blood, for the anaerobic incubation at 37 °C for up to 48 h. All colonies were counted (total counts), and morphologically different colonies were then picked for further identification.
Tryptone soy broth agar
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Tryptone soy broth agar is a general-purpose microbiological growth medium used for the cultivation and isolation of a wide range of microorganisms, including bacteria and fungi. It provides the necessary nutrients for microbial growth and development.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tryptone soy broth agar
1
Wound Microbiome Analysis Protocol
2
Soybean Seed Coat Processing and Material Sourcing
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The Black Tokyo, soybean genotype (with black seed coat) used in this study was grown at the location of the Maize Research Institute Zemun Polje (MRIZP). The whole black soybeans were separated into seed coats and dehulled beans. The separated seed coats were finely ground on a Perten 120 lab mill (Perten, Sweden) to fine powder (particle size <500 lm) and stored at À70 C.
Maltodextrin with dextrose equivalent (DE ¼ 16-19.9) produced by Cargill was kindly provided from Palco (Serbia). GA was purchased from Himedia (India), as well as M€ ueller Hinton Broth/Agar. SMP was generously donated by dairy Suboti cka mlekara (Serbia). Tryptone Soy Broth/Agar was purchased from Oxoid (Hampshire, UK). All other chemicals used for the experimental procedures were of analytical or HPLC grade and used as such without further purification.
Maltodextrin with dextrose equivalent (DE ¼ 16-19.9) produced by Cargill was kindly provided from Palco (Serbia). GA was purchased from Himedia (India), as well as M€ ueller Hinton Broth/Agar. SMP was generously donated by dairy Suboti cka mlekara (Serbia). Tryptone Soy Broth/Agar was purchased from Oxoid (Hampshire, UK). All other chemicals used for the experimental procedures were of analytical or HPLC grade and used as such without further purification.
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