The total protein concentration in BALF was measured by a dye-binding assay using a commercial kit from ThermoFisher Scientific Incorporation (Waltham, MA, USA). The levels of TGFβ1, IL-1β, osteopontin, human MMP-2 (R&D Systems, Minneapolis, MN, USA), MCP-1, IL-13, interferon (IFN)-γ, IL-6 (BD Bioscience, BD opt-EIA kits, San Diego, CA, USA), MCP-3 (Preprotec, Rocky Hill, NJ, USA), connective tissue growth factor (CTGF) (Abgent, San Diego, CA, USA), Bax (LSBio, Seattle, WA, USA), and Bcl-2 (RayBiotech Life, inc, Peachtree Corners, GA, USA) were determined using commercial immunoassay kits according to the manufacturer’s instructions. Collagen type I was determined by enzyme immunoassay using an anti-collagen type I antibody and an anti-collagen type I biotin-conjugated antibody from Rockland Immunochemicals Incorporation (Limerick, PA, USA) following the manufacturer’s instructions. The level of total PDGF was measured by immunoassay as previously described [103 (link)]. The lung hydroxyproline content was determined by a colorimetric method using a commercial kit (Hydroxyproline colorimetric assay kit, BioVision, San Francisco, CA, USA) according to the manufacturer’s instructions.
Human mmp 2
Manufactured by R&D Systems
Sourced in United States
Human MMP-2 is a recombinant human protein that represents the catalytic domain of the matrix metalloproteinase-2 enzyme. It functions as a zinc-dependent endopeptidase involved in the breakdown of extracellular matrix components.
Automatically generated - may contain errors
Lab products found in correlation
Hydroxyproline colorimetric assay kit, by Abcam (1 mentions)
Bcl2 associated x (bax), by LSBio (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
Human mmp3, by R&D Systems (1 mentions)
Human mmp1, by R&D Systems (1 mentions)
N methylmorpholine, by Merck Group (1 mentions)
2 protocols using human mmp 2
1
Comprehensive Biomarker Analysis in BALF
2
Purification and Activation of Matrix Metalloproteinases
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HPLC-grade acetonitrile was purchased from Fisher Scientific (Philipsburg, PA). Purified water was obtained using a Milli-Q water purification system (Millipore, Bedford, MA). Trifluoroacetic acid (TFA), Dimethylformamide (DMF) and N-methylmorpholine (NMM) were purchased from Sigma-Aldrich (Milwaukee, WI). α-aminoxyl-ω-methoxy, polyoxyethylene (Mw~2,000) [mPEG(2K)-ONH2] was from NOF America Corporation (Irvine, CA) and the peptide core from PolyPeptide Group (San Diego, CA).
Recombinant protease materials were used. Human MMP2, human MMP9, human MMP1, human MMP3 and human MT1-MMP (MMP14), and mouse MMP2 and mouse MMP9 were purchased from R&D Systems (Minneapolis, MN). Human MMP7 and MMP8, active human MMP13 and the catalytic domains of MT2-MMP (MMP15) and MT4-MMP (MMP17) were purchased from EMD Millipore (Billerica, MA). The catalytic domain of MMP11 was purchased from Enzo Life Sciences (Farmingdale, NY). The catalytic domain of MMP19 was purchased from Sino Biological (Beijing, China). Where necessary, pro-enzymes were activated according to the manufacturer's instructions. The MMP inhibitors GM6001 (Ilomastat) and Prinomastat were purchased from EMD Millipore and Sigma Aldrich (St. Louis, MO) respectively.
Recombinant protease materials were used. Human MMP2, human MMP9, human MMP1, human MMP3 and human MT1-MMP (MMP14), and mouse MMP2 and mouse MMP9 were purchased from R&D Systems (Minneapolis, MN). Human MMP7 and MMP8, active human MMP13 and the catalytic domains of MT2-MMP (MMP15) and MT4-MMP (MMP17) were purchased from EMD Millipore (Billerica, MA). The catalytic domain of MMP11 was purchased from Enzo Life Sciences (Farmingdale, NY). The catalytic domain of MMP19 was purchased from Sino Biological (Beijing, China). Where necessary, pro-enzymes were activated according to the manufacturer's instructions. The MMP inhibitors GM6001 (Ilomastat) and Prinomastat were purchased from EMD Millipore and Sigma Aldrich (St. Louis, MO) respectively.
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