For the Hematoxylin-Eosin (HE) and Masson trichrome staining, the heart slides were deparaffinized and rehydrated by gradient elution using xylene and ethanol, and then were stained by Hematoxylin-Eosin (Solarbio, China) and Masson trichrome staining reagent (Solarbio, China) according to the manufacturer's instructions.
Hematoxylin eosin
Manufactured by Solarbio
Sourced in China
Hematoxylin-eosin is a staining technique used in histology and pathology to visualize cellular and tissue structures. It consists of two dyes: hematoxylin, which stains nuclei blue, and eosin, which stains cytoplasm and extracellular structures pink or red.
Automatically generated - may contain errors
Lab products found in correlation
Masson trichrome, by Solarbio (3 mentions)
Masson s trichrome stain kit, by Solarbio (2 mentions)
Periodic acid schiff, by Solarbio (2 mentions)
Rm2135, by Leica (1 mentions)
Dm3000, by Leica (1 mentions)
Masson s trichrome staining, by Solarbio (1 mentions)
G1120, by Solarbio (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
He staining kit, by Solarbio (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Digital camera, by Olympus (1 mentions)
Hematoxylin, by Solarbio (1 mentions)
Trypsin, by Solarbio (1 mentions)
Biomicroscope, by Leica (1 mentions)
Dm4000b light microscope, by Leica (1 mentions)
Tissuetek cutting medium, by Sakura Finetek (1 mentions)
Oil red o, by Solarbio (1 mentions)
Masson trichrome staining reagent, by Solarbio (1 mentions)
Optic microscope, by Leica (1 mentions)
Optical microscope, by Leica (1 mentions)
19 protocols using hematoxylin eosin
1
Histological Staining of Heart Slides
2
Tissue Microarray Construction for Prostate Cancer
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All the tissue microarrays were constructed as previously described (20 (link)). In brief, one tissue core (0.6 mm) was taken from an individual paraffin-embedded PCa specimen (donor block) and arrayed precisely into a new paraffin block (35×20 mm; Zhongshan Golden Bridge Biotechnology Co. Ltd., Beijing, China) using a custom-built precision instrument (ATA-27; Beecher Instrument, Inc. Silver spring, MD, USA). Following block construction, 4 µm sections were cut from the microarray blocks using a Leica microtome (Leica RM2135; Leica Instruments GmbH, Hubloch, Germany) to support the adhesion of array elements. Overall, the tissue microarray block contained 128 donor blocks from specimens of all 128 PCa patients. The presence of prostate tissue on the arrayed specimens was verified via hematoxylin-eosin (Solarbio Biotechnology Co., Ltd., Shanghai, China) stained sectioning for the identification of pathological features associated with PCa tissue.
3
Histological Analysis of Myocardial Structure
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After 24 h of fixation with 4% paraformaldehyde, the heart tissues were dehydrated with alcohol, paraffin‐embedded, and cut into 5‐μmol/L thick slices. The slices were stained with hematoxylin–eosin (Solarbio) and Masson's trichrome (Bogoo Biotechnology, Shanghai, China). The morphology of the myocardium and collagen deposition were observed using a fluorescence microscope (IX71 Olympus, Tokyo, Japan).
4
Histological Analysis of Tissue Preservation
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To assess cell and nuclear clearance as well as preservation of collagen, hematoxylin & eosin and Masson Trichrome staining (Solarbio, China) were performed after fixation in 10% formalin, paraffin embedding, and sectioning.
5
Histological Evaluation of Osteoarthritic Cartilage
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Whole knee joints (n = 5) were fixed in 4% paraformaldehyde, decalcified, paraffin-embedded, and sectioned at 6 μm thickness for histological and immunohistochemical evaluation. The sections were stained with saffron O (Solarbio, Beijing, China), hematoxylin-eosin (Solarbio), or saffron O/solid green (Solarbio) to reveal histological changes in the cartilage. Cartilage destruction severity was graded according to the modified Mankin [40 (link)] and Osteoarthritis Research Society International (OARSI) [41 (link)] scoring systems (scoring criteria are provided in Tables S2 and S3 ).
6
Histological Evaluation of Cardiac Tissue
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Whole heart tissues were fixed in 4% paraformaldehyde (PFA) and then embedded in paraffin. The 5-μm-thick cardiac longitudinal sections were stained with Hematoxylin-Eosin (Solarbio, G1120) and Masson’s trichrome staining (Solarbio, G1340) according to manufacturer’s instructions. Tissue stainings were observed and photographed under a biological microscope (Leica, DM3000). HE staining and Masson’s trichrome staining were analyzed to evaluate the recovery of LV apex and whether fibrotic tissue was present or not after AR.
7
Histological Evaluation of Spinal Cord Tissue
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The histological evaluation was performed at 4 weeks post surgeries. The rats were anesthetized with isoflurane and transcardially perfused with 4% paraformaldehyde in PBS. Spinal cord tissue was cut into paraffin sagittal sections of 7 μm thickness. After the paraffin sections were prepared, the paraffin sections were stained with hematoxylin-eosin (Solarbio, China), as described previously [27 (link)]. Finally, the stained sections were observed under a microscope (Nikon, Japan).
8
Immunostaining Protocol for Paraffin Sections
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After dewaxing and rehydration, paraffin sections were subjected to hematoxylin–eosin staining (Solarbio, China). For immunostaining, a series of procedures, including antigen retrieval, hydrogen peroxide blocking and BSA blocking, were carried out, followed by primary antibody (listed in Additional file 3 : Table S1) incubation overnight and secondary antibody (listed in Additional file 3 : Table S1) incubation the next day. Immunochemical staining was accomplished through subsequent coloration and hematoxylin counterstaining, while immunofluorescence staining was performed with DAPI (Solarbio, China).
9
Hematoxylin-Eosin Staining of Paraffin Sections
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According to the guidance of Hematoxylin-eosin (HE) staining kit (#G1120, Solarbio), paraffin sections were routinely dewaxed to water, stained with hematoxylin, differentiated with 1% hydrochloric acid, counterstained with eosin. Finally, the slices were quickly dehydrated and transparent, and then sealed. Evos FL Auto2 was used for image acquisition.
10
Histological Evaluation of Heart Tissue
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The heart tissue samples were fixed in 4% paraformaldehyde for 24 h, dehydrated with a series of concentrations of ethanol and tetrahydrofuran, and finally embedded in paraffin. The paraffin–embedded tissue mass was cut into 5 μm slices and stained with Hematoxylin Eosin, Modified Masson's Trichrome Stain (NO: G1345, solarbio, Beijing, China)and Glycogen Periodic Acid Schiff (PAS/Hematoxylin) Stain, which was used to observe the pathological changes of heart tissue. The quantification of fibrosis and glycogen distribution in myocardial tissue was evaluated using a microscope–connected digital camera (Olympus, Nikon, Tokyo, Japan).
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