Log In Sign up
The largest database of trusted experimental protocols

Hsc007

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

HSC007 is a laboratory equipment product offered by R&D Systems. It is designed for use in research and scientific applications. The core function of this product is to [description not available].

Automatically generated - may contain errors

Lab products found in correlation

Hsc003, by R&D Systems (2 mentions) Axiovision software, by Zeiss (2 mentions) Hsc006, by R&D Systems (1 mentions) Human g csf, by Thermo Fisher Scientific (1 mentions) Murine m csf, by Thermo Fisher Scientific (1 mentions) Murine gm csf, by Thermo Fisher Scientific (1 mentions) Mouse methylcellulose complete media, by R&D Systems (1 mentions) Myelocult m5300, by STEMCELL (1 mentions) Myelocult h5100, by STEMCELL (1 mentions) Megacult c, by STEMCELL (1 mentions) M3234, by STEMCELL (1 mentions) Stemmacs hsc cfu media, by Miltenyi Biotec (1 mentions) Methocult h4435, by STEMCELL (1 mentions) Methocult h4230, by STEMCELL (1 mentions) Lysis buffer, by BioLegend (1 mentions) Collagenase, by Merck Group (1 mentions) 70 m cell strainer, by Corning (1 mentions)

23 protocols using hsc007

1

Hematopoietic Progenitor Cell Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hematopoietic CD41+ progenitor cells obtained from iPSC differentiation (8 days after start of EB culture) were seeded at a concentration of 20,000–50,000/ml in basic and complete methylcellulose medium (HSC006 with no cytokines added and HSC007 containing IL-6, erythropoietin, SCF, and IL-3, respectively; R&D Systems). HSC007 was additionally supplemented with 20 ng/ml human G-CSF and 20 ng/ml murine M-CSF, while HSC006 was supplemented with 50 ng/ml murine GM-CSF (all Peprotech). Cells were grown for 7–10 days and colonies composed of more than 50 cells were counted.
Mucci A., Kunkiel J., Suzuki T., Brennig S., Glage S., Kühnel M.P., Ackermann M., Happle C., Kuhn A., Schambach A., Trapnell B.C., Hansen G., Moritz T, & Lachmann N. (2016). Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports, 7(2), 292-305.
+ Open protocol
+ Expand
2

Quantifying Hematopoietic Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
6×104 Bone marrow cells from Foxm1fl/fl or Foxm1fl/flTie2-Cre mice were plated in triplicate in 35-mm tissue culture dishes containing Mouse Methylcellulose Complete Media (HSC007, R&D systems), after 10 days of incubation at 37 °C in 5% CO2, CFU-GEMM, CFU-GM, CFU-G, CFU-M, BFU-E were scored under an inverted microscope.
Hou Y., Li W., Sheng Y., Li L., Huang Y., Zhang Z., Zhu T., Peace D., Quigley J.G., Wu W., Zhao Y, & Qian Z. (2015). Foxm1 is essential for quiescence and maintenance of hematopoietic stem cells. Nature immunology, 16(8), 810-818.
+ Open protocol
+ Expand
3

LT-HSCs Colony Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Long‐term (LT)‐HSCs were sorted by fluorescence‐activated cell sorting (FACS) and then cultured in 96‐well cell plates using a methylcellulose‐base medium (HSC007; R&D Systems, Minneapolis, MN, USA). Ten replicate wells were prepared for each sample. We used 96‐well cell culture plate, three cells per well. Two weeks after plating, the number and sizes of colonies were counted under a microscope.
Bai L., Shi G., Yang Y., Chen W., Zhang L, & Qin C. (2018). Rehmannia glutinosa exhibits anti‐aging effect through maintaining the quiescence and decreasing the senescence of hematopoietic stem cells. Animal Models and Experimental Medicine, 1(3), 194-202.
+ Open protocol
+ Expand
4

MLL-AF9 Leukemia Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow was isolated from WT C57BL/6J mice (CD45.2). Lin−Kit+Sca-1+ cells were separated by FACS and cultured. Then, cells were transduced with MSCV-MLL-AF9-IRES-GFP retrovirus and plated in methylcellulose (R&D Systems, HSC007) for 5 d (refs. 36 (link),37 (link),62 (link)). GFP-positive cells were FACS sorted and 1.2 × 105 cells were retro-orbitally transplanted into a sublethally irradiated (650 rads) congenic C57BL/6 mouse (CD45.1) to generate an MLL-AF9 leukemic primary recipient mouse. For secondary transplantation, liver was isolated from the leukemic primary recipient mouse and GFP-positive cells were sorted by FACS. A total of 5,000 cells were injected retro-orbitally on day 1 into 8-week-old male congenic C57BL/6 (CD45.1) secondary recipient mice that had received sublethal irradiation (250 rads) on day 0. Mice were randomly assigned to treatment groups (n = 10 mice per group). Starting on day 12, treatments were administered i.p. to contralateral sides of the abdomen every other day for a total of ten doses. At 19, 26 and 33 d after injection of cells, bone marrow was aspirated to analyze leukemic infiltrates as described below. On day 34, echocardiography was performed followed by killing.
Amgalan D., Garner T.P., Pekson R., Jia X.F., Yanamandala M., Paulino V., Liang F.G., Corbalan J.J., Lee J., Chen Y., Karagiannis G.S., Sanchez L.R., Liang H., Narayanagari S.R., Mitchell K., Lopez A., Margulets V., Scarlata M., Santulli G., Asnani A., Peterson R.T., Hazan R.B., Condeelis J.S., Oktay M.H., Steidl U., Kirshenbaum L.A., Gavathiotis E, & Kitsis R.N. (2020). A small-molecule allosteric inhibitor of BAX protects against doxorubicin-induced cardiomyopathy. Nature cancer, 1(3), 315-328.
+ Open protocol
+ Expand
5

Quantifying iPSC-Derived Macrophage Colonies

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 1,500 primary bone marrow-derived lineage-negative cells, and 25,000 or 50,000 iPSC-Mφ, were seeded in a methylcellulose medium (HSC007, R&D Systems) and allowed to grow for 7 days before colonies containing more than 50 cells were counted.
Mucci A., Lopez-Rodriguez E., Hetzel M., Liu S., Suzuki T., Happle C., Ackermann M., Kempf H., Hillje R., Kunkiel J., Janosz E., Brennig S., Glage S., Bankstahl J.P., Dettmer S., Rodt T., Gohring G., Trapnell B., Hansen G., Trapnell C., Knudsen L., Lachmann N, & Moritz T. (2018). iPSC-Derived Macrophages Effectively Treat Pulmonary Alveolar Proteinosis in Csf2rb-Deficient Mice. Stem Cell Reports, 11(3), 696-710.
+ Open protocol
+ Expand
6

Clonogenic Assay for Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bulk BM cells or sorted HSPCs/HSCs/pre-LSCs were spread in 1mL cytokine-containing methylcellulose media (HSC007 for mouse, HSC003 for human, R&D systems), and plated in 35mm culture dish. For murine cells, colonies were counted and replated after 7 days.
Ueda K., Kumari R., Schwenger E., Wheat J.C., Bohorquez O., Narayanagari S.R., Taylor S.J., Carvajal L.A., Pradhan K., Bartholdy B., Todorova T.I., Goto H., Sun D., Chen J., Shan J., Song Y., Montagna C., Xiong S., Lozano G., Pellagatti A., Boultwood J., Verma A, & Steidl U. (2021). MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism. Cancer cell, 39(4), 529-547.e7.
+ Open protocol
+ Expand
7

Clonogenic Potential Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Suitable cells were plated in 96-well plates at the density of 1 cells/well, with 20 replicates per sample, in a methylcellulose-based medium (R&D, HSC007). After culturing for 14 days, the ensuing colonies were counted under a microscope. Based on the approximate cell load, the colonies were defined as large (>10,000 cells), medium (1,000–10,000) and small (<1,000).
Bai L., Lyu Y., Shi G., Li K., Huang Y., Ma Y., Cong Y.S., Zhang L, & Qin C. (2020). Polymerase I and transcript release factor transgenic mice show impaired function of hematopoietic stem cells. Aging (Albany NY), 12(20), 20152-20162.
+ Open protocol
+ Expand
8

Evaluating LSC Differentiation with ILCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
LSCs were obtained from MllPTD/WT: Flt3ITD/ITD mouse spleen and co-cultured with or without WT, IFN-γ−/− or TNF−/− ILC1s for 3 days. Cells were then plated into mouse methylcellulose complete medium (R&D, HSC007) supplied with human transferrin (200 μg/ml), recombinant human insulin (10 μg/ml), recombinant human SCF (50 ng/ml), mouse recombinant IL-3 (10 ng/ml), IL-6 (10 ng/ml), and recombinant mouse EPO (5 IU/ml). Cultures were incubated at 37°C in a humidified atmosphere of 5% CO2 for 10–14 days. Colony numbers were counted using a microscope.
Li Z., Ma R., Ma S., Tian L., Lu T., Zhang J., Mundy-Bosse B.L., Zhang B., Marcucci G., Caligiuri M.A, & Yu J. (2022). ILC1s Control Leukemia Stem Cell Fate and Limit Development of AML. Nature immunology, 23(5), 718-730.
+ Open protocol
+ Expand
9

Long-term Hematopoietic Stem Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in a methylcellulose-based medium (R&D, HSC007). For LT-HSCs, we used 96-well cell culture plates (5 cells/well), with each sample added to 10 wells. For BM and spleen cells, we used 24-well cell culture plate (104 cells/well), with each sample added to six wells. Two weeks after plating, the colonies were counted using a microscope.
Bai L., Shi G., Zhang L., Guan F., Ma Y., Li Q., Cong Y.S, & Zhang L. (2014). Cav-1 deletion impaired hematopoietic stem cell function. Cell Death & Disease, 5(3), e1140-.
+ Open protocol
+ Expand
10

Hematopoietic Progenitor Assay in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDMs or Lin−bone marrow cells were evaluated for the presence of hematopoietic progenitors capable of forming colonies in semisolid medium in response to cytokine stimulation as previously described.45 (link) Briefly, fresh Lin− bone marrow cells or BMDMs after induced differentiation into macrophages for five days were seeded into standard mouse methylcellulose media supplemented with insulin, transferrin, SCF, IL-3, IL-6, and erythropoietin (HSC007, R&D Systems, Minneapolis, MN). After seven days in culture, colonies of ≥50 cells were visible and were examined morphologically using whole plate stack images acquired using an AXIO-Z1 microscope and AXIO-vision software (Zeiss, Jena, Germany) to identify and enumerate burst-forming erythroid progenitors (BFU-E), colony-forming myeloid progenitors (CFU-GM), and the multi-potential progenitors (CFU-GEMM).
Suzuki T., Arumugam P., Sakagami T., Lachmann N., Chalk C., Sallese A., Abe S., Trapnell C., Carey B., Moritz T., Malik P., Lutzko C., Wood R.E, & Trapnell B.C. (2014). Pulmonary Macrophage Transplantation Therapy. Nature, 514(7523), 450-454.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!