Glioma cell lines U87 and A172 were supplied by the American Type Culture Collection and maintained in DMEM (Invitrogen, Carlsbad, CA) and supplemented with 10% FBS (Hyclone, Logan, UT) and 1% penicillin/streptomycin (Invitrogen). Primary glioma cell and primary astrocyte were isolated from GBM sample and non-tumoral brain tissue. miR-16 mimic, anta-miR-16, sc-miRNA and sc-antagomir were purchased from RIBOBIO (CHN) and transfected in accordance with the manufacturer’s instruction. Details are described in supplementary methods.
Mir 16 mimic
Manufactured by RiboBio
Sourced in China
The MiR-16 mimic is a laboratory product designed to mimic the function of the microRNA miR-16. MiR-16 is a small, non-coding RNA molecule that plays a role in regulating gene expression. The MiR-16 mimic is intended to be used as a research tool to study the biological functions and mechanisms of miR-16 in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Inhibitor control, by RiboBio (1 mentions)
Hpde6 c7, by American Type Culture Collection (1 mentions)
Mir 15a mimic, by RiboBio (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Sirnas, by RiboBio (1 mentions)
Control sirna, by RiboBio (1 mentions)
Mimic control, by RiboBio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Scc25, by Thermo Fisher Scientific (1 mentions)
Cal27, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pcdna3, by Thermo Fisher Scientific (1 mentions)
4 protocols using mir 16 mimic
1
Glioma Cell Characterization and miR-16 Modulation
2
Mimic Preparation and Handling
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The synthetic miR-16 mimic and the mimic negative control (NC) were purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China). The synthetic samples were first centrifuged at 12,000 × g for 20 min at 25°C following dilution with 250 µl diethyl-pyrocarbonate-treated water as the mother liquor per 5 nmol miRNA. The samples were subpackaged and stored at −20°C until further use. The miR sequences were as follows: miR-16 mimic, 5′-UAGCAGCACGUAAUAUUGGCGCCAAUAUUUACGUGCUGCUAUU-3′; and miR-16 mimic NC, 5′-UUCUCCGAACGUGUCACGUTTACGUGACACGUUCGGAGAATT-3′.
3
DLEU2 and SMAD2 Regulation in Pancreatic Cells
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Human PC cell lines AsPC‐1 and PaCa‐2 and human pancreatic duct epithelial cell line HPDE6‐C7 were obtained from ATCC (Rockville, MD, USA). The cells were cultured in RPMI‐1640 or DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS. The DLEU2 expression plasmid (pcDNA3.1‐DLEU2) containing DLEU2 (variant uc001vdy.3), and the SMAD2 expression plasmid pcDNA3.1‐SMAD2 were constructed by GenePharma (Shanghai, China). In addition, siRNAs targeting DLEU2, siRNAs targeting SMAD2, control siRNA, miR‐455 mimic, miR‐15a mimic, miR‐16 mimic, control mimic, miR‐455 inhibitor, and control inhibitor were prepared by RiboBio (Guangzhou, China). Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) was used for cell transfection following the manufacturer's instructions.
4
Overexpression of miR-16 and its targets in OSCC
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Human immortalized oral epithelial cells (HIOEC) and five human OSCC cell lines (SCC‐4, SCC‐25, HN‐6, CAL‐27, and TCA‐83) were purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, China). Cells were maintained in Dulbecco modified Eagle’s medium (Gibco, Invitrogen Corporation, Carlsbad, CA) with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin at 37℃ in a standard humidified incubator containing 5% CO2.
The miR‐16 mimic, the miR‐16 inhibitor, and their corresponding negative control (miR‐16 mimic NC and miR‐16 inhibitor NC) were purchased from RiboBio Co., Ltd. (Guangzhou, China). SCC‐25 and CAL‐27 cells were transfected with the above mimic/inhibitor or corresponding negative control for 48 hr using Lipofectamine 2000 (Life Technologies, Waltham, MA) according to the manufacturer’s protocol. For the overexpression of AKT3 and BCL2L2, the cDNA of AKT3 or BCL2L2 was cloned into the mammalian expression vector pcDNA3.1 (Invitrogen, Waltham, MA), respectively, to construct two overexpressed vectors; pcDNA3.1‐AKT3 and pcDNA3.1‐BCL2L2. The pcDNA3.1 was used as a NC.
The miR‐16 mimic, the miR‐16 inhibitor, and their corresponding negative control (miR‐16 mimic NC and miR‐16 inhibitor NC) were purchased from RiboBio Co., Ltd. (Guangzhou, China). SCC‐25 and CAL‐27 cells were transfected with the above mimic/inhibitor or corresponding negative control for 48 hr using Lipofectamine 2000 (Life Technologies, Waltham, MA) according to the manufacturer’s protocol. For the overexpression of AKT3 and BCL2L2, the cDNA of AKT3 or BCL2L2 was cloned into the mammalian expression vector pcDNA3.1 (Invitrogen, Waltham, MA), respectively, to construct two overexpressed vectors; pcDNA3.1‐AKT3 and pcDNA3.1‐BCL2L2. The pcDNA3.1 was used as a NC.
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