PE was purchased from Tokyo Chemical Industry (P0398). The autophagy inhibitor CQ was obtained from Sigma‐Aldrich, St Louis, MO, USA (C6628) and was applied to cardiomyocytes at a concentration of 10 μM 16 . Protein G‐Agarose was purchased from Roche (Roche Diagnostics, Mannheim, Germany). For the Western blotting detection of specific proteins, the following primary antibodies obtained from Cell Signaling Technology (Danvers, MA, USA) were used: anti‐Atg7 (#2631), anti‐AMPK (Thr172) (#2531), anti‐AMPK (#2532), antiphosphorylated mTOR (Ser2448) (#5536), antiphosphorylated p70S6K (Thr389) (#9234), anti‐p70S6K (#2708), antiphosphorylated Akt (Ser473) (#4058), anti‐Akt (#9272) and anti‐GAPDH (#2118) antibodies. Anti‐Sestrin 1 antibody was obtained from Abcam, Cambridge, UK (ab134091). Anti‐LC3B antibody was obtained from Novus Biologicals, Littleton, CO, USA (NB100‐2220). Anti‐p62 antibody was purchased from Sigma‐Aldrich (P0068). Antivinculin antibody was purchased from Sigma‐Aldrich (V9264). Anti‐cathepsin D antibody was obtained from Santa Cruz Biotechnology, Dallas, TX, USA (sc‐6486).
Anti cathepsin d antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-cathepsin D antibody is a laboratory reagent used to detect the presence and quantify the levels of cathepsin D protein in biological samples. Cathepsin D is an aspartic protease enzyme involved in various cellular processes. This antibody can be utilized in techniques such as western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) to study the expression and distribution of cathepsin D in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti phosphorylated mtorser2448, by Cell Signaling Technology (1 mentions)
Antiphosphorylated akt ser473, by Cell Signaling Technology (1 mentions)
Protein g agarose, by Roche (1 mentions)
Anti vinculin antibody, by Merck Group (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti p70s6k, by Cell Signaling Technology (1 mentions)
Anti atg7, by Cell Signaling Technology (1 mentions)
Anti ampk thr172, by Cell Signaling Technology (1 mentions)
Antiphosphorylated p70s6k thr389, by Cell Signaling Technology (1 mentions)
Anti lc3b antibody, by Novus Biologicals (1 mentions)
Anti p62 antibody, by Merck Group (1 mentions)
Anti ampk, by Cell Signaling Technology (1 mentions)
Anti rabbit igg antibody, by Bio-Rad (1 mentions)
Anti tfeb antibody, by Santa Cruz Biotechnology (1 mentions)
Anti gpr81 antibody, by Novus Biologicals (1 mentions)
2 protocols using anti cathepsin d antibody
1
Autophagy Modulation in Cardiomyocytes
2
Western Blot Analysis of Protein Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The samples were lysed in radioimmunoprecipitation assay buffer and vortexed three times for 30 s each. After centrifugation at 13,000g, the lysates were mixed with sample buffer and separated on 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels. Proteins on SDS-PAGE gels were transferred to PVDF membranes (Immobilon) and then blocked for 1 h in 3% BSA in Tris-buffer saline with Tween-20. Membranes were treated with a human anti-IFN-α/βRα mouse monoclonal antibody, anti-TFEB antibody, anti-cathepsin D antibody (Santa Cruz Biotechnology), and anti-GPR81 antibody (Novus Biological) overnight at 4 °C. Secondary antibodies were anti-mouse immunoglobulin G (IgG) or anti-rabbit IgG antibodies (BioRad) coupled with horseradish peroxidase.
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