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Dihydroethidium assay kit

Manufactured by Beyotime
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Sourced in China

The Dihydroethidium Assay Kit is a fluorometric detection system designed to measure the intracellular levels of superoxide. It contains dihydroethidium, a cell-permeable fluorogenic probe that is oxidized by superoxide to form the fluorescent product ethidium. The kit provides a simple and convenient method for quantifying superoxide production in various biological samples.

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Lab products found in correlation

Ix71 fluorescence microscope, by Olympus (1 mentions) Gsh assay kit, by Nanjing Jiancheng (1 mentions) Eclipse ti u fluorescent microscope, by Nikon (1 mentions)

Close spelling variants of this product

Dihydroethidium (DHE) assay kit Dihydroethidium kit Dihydroethidium

2 protocols using dihydroethidium assay kit

1

Quantifying Intracellular GSH and ROS

Check if the same lab product or an alternative is used in the 5 most similar protocols
The intracellular concentration of GSH was assessed utilizing the GSH Assay Kit (Nanjing Jiancheng Bioengineering Institute, A006-2-1) based on the instructions provided by the manufacturer. Dihydroethidium Assay Kit (Beyotime, S0063) was used to detect intracellular ROS levels. The observations were made on an Olympus IX71 fluorescence microscope in a blinded manner and the average fluorescence intensity was determined by analyzing the images using image J software (v.1.8.0).
Zhang Z., Huang Y., Li S, & Hong L. (2024). Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer. Journal of Cancer, 15(2), 383-400.
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2

Quantifying Cellular ROS using DHE

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Dihydroethidium assay kit (Beyotime, Shanghai, China) was used to detect the level of ROS in Hela cells. The working solution Dihydroethidium (5 μM) was added to the Hela cells for 30 min after the treatments. Imaging was performed with a Nikon Eclipse Ti-U fluorescent microscope.
Chen K., Wang J., Guo L., Wang J., Yang L., Hu T., Zhao Y., Wang X, & Zhu Y. (2023). Lactobacillus johnsonii L531 Ameliorates Salmonella enterica Serovar Typhimurium Diarrhea by Modulating Iron Homeostasis and Oxidative Stress via the IRP2 Pathway. Nutrients, 15(5), 1127.
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