Log In Sign up
The largest database of trusted experimental protocols

Optilab differential refractive index detector

Manufactured by Wyatt Technology
Visit Supplier
Sourced in United States

The Optilab differential refractive index (dRI) detector is a lab equipment product designed to measure the refractive index of a sample. It operates by comparing the refractive index of the sample to a reference material, providing a sensitive and accurate measurement of the sample's composition.

Automatically generated - may contain errors

Lab products found in correlation

Dawn mals detector, by Wyatt Technology (3 mentions) Astra software, by Wyatt Technology (3 mentions) Minidawn mals detector, by Wyatt Technology (2 mentions) Tskgel g4000swxl analytical column, by Tosoh (1 mentions) Superdex 200 increase 10 300 column, by GE Healthcare (1 mentions) Astra 7, by Wyatt Technology (1 mentions) Akta pure, by GE Healthcare (1 mentions) Superdex 75 increase 10 300 gl column, by Cytiva (1 mentions) Astra 8, by Wyatt Technology (1 mentions) Superdex 200 increase 10 300 gl column, by Cytiva (1 mentions) Akta pure fplc, by Cytiva (1 mentions) Minidawn light scattering detector, by Wyatt Technology (1 mentions)

Close spelling variants of this product

Optilab T-rEX differential refractive index detector Optilab rEX differential refractive index detector Optilab refractive index detector Differential refractive index detector Refractive index detector

7 protocols using optilab differential refractive index detector

1

Characterizing S. enterica WbaP in SMALP

Check if the same lab product or an alternative is used in the 5 most similar protocols
A 50 μL aliquot of 0.5 mg/mL S. enterica WbaP in SMALP was injected onto a WTC-030 fused silica column pre-equilibrated with HEPES buffered saline pH 7.5 (HBS). Eluate was flowed through a DAWN MALS detector (Wyatt) and an Optilab differential refractive index detector (Wyatt). Data were analyzed using ASTRA software (Wyatt), and the system was pre-calibrated with a BSA standard.
Dodge G.J., Anderson A.J., He Y., Liu W., Viner R, & Imperiali B. (2023). Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle. bioRxiv.
+ Open protocol
+ Expand
2

SMALP-Encapsulated S. enterica WbaP Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
A 50 μL aliquot of 0.5 mg/mL S. enterica WbaP in SMALP was injected onto a WTC-030 fused silica column pre-equilibrated with HEPES buffered saline pH 7.5 (HBS). Eluate was flowed through a DAWN MALS detector (Wyatt) and an Optilab differential refractive index detector (Wyatt). Data were analyzed using ASTRA software (Wyatt), and the system was pre-calibrated with a BSA standard.
Dodge G.J., Anderson A.J., He Y., Liu W., Viner R, & Imperiali B. (2024). Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle. eLife, 12, RP91125.
+ Open protocol
+ Expand
3

Size Exclusion Chromatography for Molecular Weight

Check if the same lab product or an alternative is used in the 5 most similar protocols
Size exclusion chromatography, using a model 1260 Infinity autosampler and pump (Agilent Technologies, Germany), coupled to a HELEOS II Multiangle laser light-scattering photometer (MALLS; Wyatt Technology, Santa Barbara, CA, USA), a Viscostar II differential pressure viscometer (DP; used to estimate intrinsic viscosity), and an Optilab differential refractive index detector (dRI; Wyatt Technology, Santa Barbara, CA, USA), was used to determine number average (Mn) and weight average (Mw) molecular weight and intrinsic viscosity [η] (10 (link)). A minimum of three replicates were run, and the data were statistically analyzed by two-way ANOVA and means separated where appropriate using Tukey's multiple comparison (GraphPad Prizm, version 4.00 for Windows, GraphPad Software, San Diego, CA, USA, www.graphpad.com).
Dorado C., Cameron R.G., Manthey J.A., Bai J, & Ferguson K.L. (2021). Analysis and Potential Value of Compounds Extracted From Star Ruby, Rio Red, and Ruby Red Grapefruit, and Grapefruit Juice Processing Residues via Steam Explosion. Frontiers in Nutrition, 8, 691663.
+ Open protocol
+ Expand
4

Analytical HPLC Characterization of Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
The system consisted of an Agilent 1200 HPLC system, a DAWN MALS detector (Wyatt Technology), an Optilab differential Refractive Index (dRI) detector (Wyatt Technology), and a TSKgel G4000SWxl analytical column (Tosoh). PBS was used as the running buffer with a flow rate of 0.8 mL/min. Data acquisition was performed using Astra software (Wyatt Technology).
Liu J., Mao F., Chen J., Lu S., Qi Y., Sun Y., Fang L., Yeung M.L., Liu C., Yu G., Li G., Liu X., Yao Y., Huang P., Hao D., Liu Z., Ding Y., Liu H., Yang F., Chen P., Sa R., Sheng Y., Tian X., Peng R., Li X., Luo J., Cheng Y., Zheng Y., Lin Y., Song R., Jin R., Huang B., Choe H., Farzan M., Yuen K.Y., Tan W., Peng X., Sui J, & Li W. (2023). An IgM-like inhalable ACE2 fusion protein broadly neutralizes SARS-CoV-2 variants. Nature Communications, 14, 5191.
+ Open protocol
+ Expand
5

Structural analysis of FoxP variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
NusA-fused FoxP3 variants, FoxP1RBR-forkhead, FoxP2RBR-forkhead and FoxP4RBR-forkhead were analyzed with Superdex 200 Increase 10/300 column (GE Healthcare), which was connected to a miniDAWN MALS detector (Wyatt Technology) and an Optilab differential refractive index (dRI) detector (Wyatt Technology). The buffer (20 mM Tris-Hcl pH 7.5, 150 mM Nacl, 2 mM DTT) was used for size-exclusion chromatography. The AKTA pure (GE Healthcare)’s UV 280 nm absorbance signal was used for concentration detection. The Optilab differential refractive index (dRI) detector measured dRI data for additional concentration analysis. Data analysis and MW calculations were performed using the ASTRA7.3.1 software (Wyatt Technology).
Leng F., Zhang W., Ramirez R.N., Leon J., Zhong Y., Hou L., Yuki K., van der Veeken J., Rudensky A.Y., Benoist C, & Hur S. (2022). The transcription factor FoxP3 can fold into two dimerization states with divergent implications for regulatory T cell function and immune homeostasis. Immunity, 55(8), 1354-1369.e8.
+ Open protocol
+ Expand
6

SEC-MALS Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
For SEC‐MALS measurements, a Superdex 75 Increase 10/300 GL column (Cytiva Life Sciences) coupled to an ÄKTA pure™ protein purification system was used, which was further connected to a miniDAWN MALS detector and an Optilab differential refractive index (dRI) detector (Wyatt Technology). Measurements were performed at room temperature using buffer C with the addition of 0.02% sodium azide, 0.8 mL min−1 flow rate, and protein concentrations of 1, 2, and 3 mg mL−1 with an injection volume of 100 μL. Reproducibility of the SEC‐MALS runs was ensured by obtaining identical results in the measured BSA (bovine serum albumin) samples (Pierce) at 2 mg mL−1 as the first and last sample of each day's measurements. The first BSA injection was used for detector normalization, peak alignment, and band broadening. The dRI signal was used as a concentration source for molar mass determination in all cases. Data were collected and analyzed using ASTRA 8.0.2.5 software (Wyatt Technology).
Koch J., Romero‐Romero S, & Höcker B. (2024). Stepwise introduction of stabilizing mutations reveals nonlinear additive effects in de novo TIM barrels. Protein Science : A Publication of the Protein Society, 33(3), e4926.
+ Open protocol
+ Expand
7

Oligomeric State Determination of McpZ

Check if the same lab product or an alternative is used in the 5 most similar protocols
Size exclusion chromatography coupled with multi angle light scattering (SEC-MALS) was employed to determine the oligomeric state of McpZPD in solution. To this end, 40 μM McpZPD protein in buffer C was applied as a 100-μl injection to a Superdex 200 Increase 10/300 GL column (Cytiva) pre-equilibrated in the same buffer at a flow rate of 0.3 ml/min by an AKTA pure FPLC (Cytiva). The eluate was passed through an inline miniDAWN light scattering detector and Optilab differential refractive index (dRI) detector (Wyatt Technology Corporation). Calculations of the molecular mass from the intensity of scattered light and dRI measurements were performed using ASTRA 8.1 (Wyatt Technology Corporation). The SEC-MALS data are summarized in Fig. S1c.
Basu D., Williams C.B, & Allen P.M. (1998). In vivo antagonism of a T cell response by an endogenously expressed ligand. Proceedings of the National Academy of Sciences of the United States of America, 95(24), 14332-14336.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!