C3b9 hybridoma cell line

Manufactured by Merck Group

The C3B9 hybridoma cell line is a laboratory tool used for the production of monoclonal antibodies. It is a fusion of mouse myeloma cells and mouse B cells, which allows for the continuous production of a specific antibody. The core function of this cell line is to serve as a source of monoclonal antibodies for research and diagnostic applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (2 mentions) Dmi8 epifluorescence microscope, by Leica (1 mentions) F actin, by Santa Cruz Biotechnology (1 mentions) Fluoview fv1000 confocal microscope, by Olympus (1 mentions)

2 protocols using c3b9 hybridoma cell line

Primary Cilia Imaging and Analysis

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For primary cilia imaging and analysis, MLO‐Y4 cells cultured on collagen I‐coated glass‐bottom 24‐well plates (Mattek) were fixed in 10% formalin and treated with anti‐acetylated α‐tubulin primary antibody, 1:1, from a C3B9 hybridoma cell line (Sigma–Aldrich). Cilia were visualized with Alexa‐Fluor 488 secondary antibody, 1:1000 (Life Technologies) and imaged with a 100× oil objective on a Leica DMi8 epifluorescence microscope. Nuclei were stained with DAPI (Life Technologies), and F‐actin was stained with phalloidin (Santa Cruz Biotech). Cell area, and circularity, and cilia incidence and length were analyzed using ImageJ software.

Verbruggen S.W., Nolan J., Duffy M.P., Pearce O.M., Jacobs C.R, & Knight M.M. (2023). A Novel Primary Cilium‐Mediated Mechanism Through which Osteocytes Regulate Metastatic Behavior of Both Breast and Prostate Cancer Cells. Advanced Science, 11(2), 2305842.

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Imaging and Analyzing Primary Cilia

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For primary cilia imaging and analysis, cells cultured on collagen I-coated glass were fixed in 10% formalin and treated with anti-acetylated α-tubulin primary antibody, 1:1, from a C3B9 hybridoma cell line (Sigma). Cilia were visualized with Alexa-Fluor 488 secondary antibody, 1:1000, (Life Technologies), and imaged with a 100× oil objective on an Olympus Fluoview FV1000 confocal microscope. Nuclei were stained with DAPI (Life Technologies). Cilia lengths were analyzed using Image J.

Spasic M, & Jacobs C.R. (2017). Lengthening primary cilia enhances cellular mechanosensitivity. European cells & materials, 33, 158-168.

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