Certified neonicotinoid standards, acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiamethoxam, and thiacloprid, were purchased from FUJIFILM Wako Pure Chemical Corporation. (Osaka, Japan). A standard solution containing ISs of seven neonicotinoids (acetamiprid-d3, clothianidin-d3, dinotefuran-d3, imidacloprid-d4, nitenpyram-d3, thiamethoxam-d3, and thiacloprid-d4), at a concentration of 10 µg/mL in acetonitrile were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). The chemical structures of the seven neonicotinoid pesticides and their stable isotope-labeled surrogates are shown in Fig. 1 . acetonitrile, methanol, and ultrapure water purchased from Wako were of the pesticide-analysis grade and were used for the extraction and cleanup processes during the analysis.
Dinotefuran
Manufactured by Fujifilm
Sourced in Japan
Dinotefuran is a chemical compound used in laboratory equipment. It functions as a pesticide, specifically targeting certain insects. The compound is designed to be effective in controlling and managing insect populations within controlled environments.
Automatically generated - may contain errors
Lab products found in correlation
Clothianidin, by Fujifilm (4 mentions)
Nitenpyram, by Fujifilm (2 mentions)
Imidacloprid, by Fujifilm (2 mentions)
Thiamethoxam, by Fujifilm (2 mentions)
Thiacloprid, by Fujifilm (2 mentions)
Methanol, by Fujifilm (2 mentions)
Acetonitrile, by Fujifilm (2 mentions)
Imidacloprid, by AccuStandard (2 mentions)
Thiamethoxam, by AccuStandard (2 mentions)
Acetamiprid, by AccuStandard (2 mentions)
Desmethyl acetamiprid, by Merck Group (2 mentions)
Acetamiprid, by Fujifilm (1 mentions)
Analytical grade ammonium formate, by Fujifilm (1 mentions)
Lc ms grade methanol, by Fujifilm (1 mentions)
Nitenpyram, by AccuStandard (1 mentions)
Thiacloprid, by AccuStandard (1 mentions)
Horseradish peroxidase hrp, by Toyobo (1 mentions)
Boscalid, by Fujifilm (1 mentions)
Anti nitenpyram moab, by Horiba (1 mentions)
5 protocols using dinotefuran
1
Quantitative Analysis of Neonicotinoids
2
Quantitative Analysis of Pesticide Residues
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Analytical standards of dinotefuran (purity 99.8%), fludioxonil (purity 100%), and pyridalyl (purity 99.2%) were purchased from FUJIFILM Wako Pure Chemical (Japan). Pesticide analysis-grade acetone, acetonitrile, and methanol; LC-MS-grade methanol; and analytical-grade ammonium formate and formic acid were purchased from FUJIFILM Wako Pure Chemical. Water used in the experiments was purified using a PURELAB Flex-UV system (Veolia Water Technologies, France).
Standard stock solutions (200 mg/L) of each pesticide were prepared separately with acetone. An aliquot of the dinotefuran stock solution was diluted with methanol/water (1 : 4, v/v) to prepare standard solutions with concentrations ranging from 0.05 to 2 µg/L. Aliquots of the fludioxonil and pyridalyl stock solutions were mixed and diluted with methanol to prepare standard solutions with concentrations ranging from 0.05 to 2 µg/L. These standard solutions were used for preparing calibration curves.
Standard stock solutions (200 mg/L) of each pesticide were prepared separately with acetone. An aliquot of the dinotefuran stock solution was diluted with methanol/water (1 : 4, v/v) to prepare standard solutions with concentrations ranging from 0.05 to 2 µg/L. Aliquots of the fludioxonil and pyridalyl stock solutions were mixed and diluted with methanol to prepare standard solutions with concentrations ranging from 0.05 to 2 µg/L. These standard solutions were used for preparing calibration curves.
3
Quantitative analysis of neonicotinoids
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Acetamiprid, imidacloprid, nitenpyram, thiacloprid, and thiamethoxam were obtained from AccuStandard (New Haven, CT, USA). Clothianidin, dinotefuran, and thiacloprid amide were obtained from Wako Pure Chemical Industries (Osaka, Japan). Desmethyl-Acetamiprid and desmethyl-thiamethoxam were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 was obtained from Hayashi Pure Chemicals (Osaka, Japan). Acetamiprid-d3, Clothianidin-d3, imidacloprid-d4, and thiamethoxam-d4 were obtained from Dr. Ehrenstorfer (Augsburg, Germany). dinotefuran-d3 and thiacloprid-d4 were obtained from @rtMolecule (Poitiers, France) and C/D/N Isotopes (Pointe-Claire, Canada), respectively.
4
Pesticide Detection Assay Development
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Boscalid, benalaxyl, fenhexamid, tecloftalam, clothianidin, nitenpyram, imidacloprid, dinotefuran, thiacloprid, thiamethoxam were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Acetamiprid was purchased from Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). Bovine serum albumin (BSA: Prod. No. A7888) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Horseradish peroxidase (HRP) was purchased from Toyobo Co., Ltd. (Osaka, Japan). The anti-Boscalid monoclonal antibody (MoAb) was prepared as described previously. 20 The anti-clothianidin MoAb and anti-nitenpyram MoAb were provided from Horiba Ltd. (Kyoto, Japan). 18 All other chemicals and reagents used were of analytical grade, and purchased from Wako Pure Chemical Industries, Ltd. or Nacalai Tesque, Inc. (Kyoto, Japan).
5
Quantification of Neonicotinoid Pesticides
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We analyzed six compounds, five of which were mother compounds (Acetamiprid, Clothianidin, dinote-furan, imidacloprid, and thiamethoxam) and the other a metabolite (desmethyl-Acetamiprid). imidacloprid currently accounts for approximately 41.5% of the global neonicotinoid market in terms of sales, and this is followed by thiamethoxam (23.8%), Clothianidin (16.7%), and Acetamiprid (10.5%) 12) . dinotefuran accounts for approximately 3% of the global neonicotinoid market 12) . The major neonicotinoid compounds were therefore included in this study.
Acetamiprid, imidacloprid, and thiamethoxam were obtained from AccuStandard (New Haven, CT, USA). Clothianidin and dinotefuran were purchased from Wako Pure Chemical Industries (Osaka, Japan). Desmethyl-Acetamiprid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Isotope-labeled Acetamiprid (Acetamiprid-d6) was purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetamiprid-d3, Clothianidin-d3, imidacloprid-d4, and thiamethoxam-d4 were obtained from Dr. Ehrenstorfer Inc. (Augsburg, Germany). dinotefuran-d3 was purchased from @rtMolecule (Poitiers, France). All reagents were of analytical grade.
Acetamiprid, imidacloprid, and thiamethoxam were obtained from AccuStandard (New Haven, CT, USA). Clothianidin and dinotefuran were purchased from Wako Pure Chemical Industries (Osaka, Japan). Desmethyl-Acetamiprid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Isotope-labeled Acetamiprid (Acetamiprid-d6) was purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetamiprid-d3, Clothianidin-d3, imidacloprid-d4, and thiamethoxam-d4 were obtained from Dr. Ehrenstorfer Inc. (Augsburg, Germany). dinotefuran-d3 was purchased from @rtMolecule (Poitiers, France). All reagents were of analytical grade.
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