5260 auto sampler

A voltammetric behavior experiment was performed using a 621E electrochemical analyzer (CH Instruments, Austin, TX, USA). The identification and quantification of cannabinoids were conducted using a high performance liquid chromatography and electrochemical detection (HPLC-EC) system consisting of a 5160 pump and a 5260 auto sampler (Hitachi, Tokyo, Japan) coupled with a LC-4C amperometric detector (BAS, West Lafayette, IN, USA). MAE experiments were performed using a MARS 5 microwave system (CEM, Matthews, NC, USA). UAE experiments were performed using a 3210 ultrasonic system (Branson, Danbury, CT, USA). SFE experiments were performed using a Spe-ed SFE supercritical fluid system consisting of an SFE oven module, SFE pump module, and SFE control and collection module (Applied Separations, Allentown, PA, USA) coupled with air compressors (JW, Taichung, Taiwan). A D-606 cooling bath (Deng Yng, New Taipei, Taiwan) was applied for HRE, SE, and SFE experiments. Microstructure analysis was performed using a Quanta 200 environmental scanning electron microscope (SEM, FEI, Hillsboro, OR, USA). A 108 Sputter Coater (Cressington, Watford, Hertfordshire, UK) was used for gold coating.

The experimental design was adapted from a previous study [28 (link)]. A high-performance liquid chromatograph (HPLC; Hitachi, Tokyo, Japan) was employed to analyze the ability of lutein to capture the metaphase dicarbonyl compound MG. For each grade, 0.5 mL of different concentrations of PNPs (0.5, 1, and 1.5 mg/mL) was dissolved in water and mixed with 0.5 mL of 2 mM MG and 0.5 mL of 12 mM o-phenylenediamine. Samples were then reacted at 37 °C for 30 min for derivatization. Analysis was performed after filtering with a 0.22 μm syringe filter. The HPLC system (Hitachi 5110 pump, Hitachi 5260 Auto Sampler, Hitachi 5260 auto-degasser, and Hitachi 5420 UV-VIS detector, Tokyo, Japan) was equipped with a C18 column (250 nm × 4.6 mm, ID: 5 µm, Code No.: 38145-21, Nacali Tesque). The column was flushed with a mixture of 4:60.15% acetic acid/water–methanol at a flow rate of 0.8 mL/min. The injection volume was 20 μL, and the wavelength used for detection was 315 nm. AG was employed as a positive control to compare the residence time of the analysis peak of the standard and the sample and to calculate the ratio of the peak area of the sample relative to the peak area of the standard. The MG-trapping percentage was calculated using the following formula: $$\mathrm{MG-trapping} (\%)=[100-\frac{Amount of M{G}_{\left(sample\right)}- Amount of M{G}_{\left(control\right)}}{ Amount of M{G}_{\left(control\right)}}]\times 100\%$$