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Inorganic pyrophosphate ppi

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Inorganic pyrophosphate (PPi) is a chemical compound used in various laboratory applications. It functions as a substrate and cofactor in enzymatic reactions. PPi is a byproduct of many biosynthetic processes, particularly those involving the hydrolysis of nucleoside triphosphates.

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Lab products found in correlation

Udp glc, by Merck Group (1 mentions) Glc 1 p, by Merck Group (1 mentions) Gal 1p, by Merck Group (1 mentions) Gdp glc, by Merck Group (1 mentions) Fru 1 6 bisp, by Merck Group (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Etidronate, by Merck Group (1 mentions) Na2hpo4 nah2po4, by Merck Group (1 mentions)

2 protocols using inorganic pyrophosphate ppi

1

Enzymatic Assays for Carbohydrate Metabolism

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Glc-1P, Gal-1P, GlcN-1P, ATP, ITP, UTP, CTP, GTP, ADP-Glc, UDP-Glc, GDP-Glc, inorganic pyrophosphate (PPi) and Fru-1,6-bisP were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of the highest quality available.
Ebrecht A.C., Solamen L., Hill B.L., Iglesias A.A., Olsen K.W, & Ballicora M.A. (2017). Allosteric Control of Substrate Specificity of the Escherichia coli ADP-Glucose Pyrophosphorylase. Frontiers in Chemistry, 5, 41.
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2

Calcification Induction and Inhibitors in VICs

Cited 1 time
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SAVIC and RAVIC cells were seeded in growth media in multi-well plates at a density of 1.11×104 cells/cm2. Calcification was induced as described previously (22 (link),25 (link)). Cells were grown to 80% confluence (Day 0), before treating with control (1.05 mM Ca/0.95 mM Pi) or test media: 1.5 to 3.6 mM calcium (Ca) and/or 1.5 to 2.5 mM phosphate (Pi). CaCl2 and Na2HPO4/NaH2PO4 (Sigma-Aldrich, Dorset, UK) were used to supplement ionic calcium and phosphate in the media. To study the effect of calcification inhibitors and bisphosphonates on VIC calcification, SAVICs were exposed to inorganic pyrophosphate (PPi) and etidronate (both 0.1 mM; Sigma-Aldrich). Cells were incubated for up to 7 days in a humidified atmosphere of 95% air/5% CO2, and the medium was changed every second/third day.
Tsang H.G., Cui L., Farquharson C., Corcoran B.M., Summers K.M, & Macrae V.E. (2017). Exploiting novel valve interstitial cell lines to study calcific aortic valve disease. Molecular Medicine Reports, 17(2), 2100-2106.
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