A total of 1000 μg protein was immunoprecipitated with mouse anti-BAX antibody (6A7, Sigma) or mouse anti-BAK (TC-100, Merck Millipore) and 5 μl Dynabeads Pan Mouse IgG (Dako, Hamburg, Germany). The precipitate was analyzed by Western blotting using the BAX NT antibody (Merck Millipore) or BAK antibody (BD, Bioscience).
Mouse anti bax antibody 6a7
Manufactured by Merck Group
Sourced in Germany
The Mouse anti-BAX antibody (6A7) is a laboratory reagent used in research applications. It specifically binds to the BAX protein, which is involved in the regulation of apoptosis (programmed cell death). This antibody can be used in various immunoassay techniques to detect and study the BAX protein.
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3 protocols using mouse anti bax antibody 6a7
1
Immunoprecipitation and Western Blotting of BAX and BAK
2
Detection of Active BAX Protein
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For detection of active BAX, cells were lysed in CHAPS lysis buffer (10 mM HEPES (pH 7.4), 150 mM NaCl, 1% CHAPS) as previously described [50 (link)]. Briefly, 1000 μg protein were incubated overnight at 4°C with 5 μg mouse anti-BAX antibody (6A7, Sigma) followed by addition of 10 μL Dynabeads Protein G (Invitrogen) and further incubation for 2h at 4°C. Dynabeads were washed with lysis buffer and BAX levels were analyzed by immunoblotting using rabbit anti-BAX (1:1000; 5023, Cell Signaling).
3
Detection of Active Bax and Bak
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For detection of active Bax or Bak, cells were lysed in CHAPS lysis buffer (10 mM HEPES (pH 7.4); 150 mM NaCl; 1% CHAPS) as previously described [29 (link)]. Briefly, 700-1000 μg protein was immunoprecipitated and incubated overnight at 4°C with 2 μg/ml mouse anti-Bax antibody (6A7, Sigma) or anti-Bak antibody (Ab-1; Calbiochem) and 10 μl pan-mouse IgG Dynabeads (Dako, Hamburg, Germany) and washed with CHAPS buffer. The precipitate was analyzed for Bax and Bak expression by Western blotting using the Bax NT antibody (Merck) or Bak antibody (BD Biosciences).
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