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Glucose oxidase

Manufactured by MP Biomedicals

Glucose oxidase is an enzyme that catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. It is commonly used in various laboratory applications, including the detection and quantification of glucose in biological samples.

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2 protocols using glucose oxidase

1

Fluorescent Nanoparticle Imaging Protocol

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Sylgard 184 PDMS base and curing agent were from Dow Corning (Midland, MI). Quantum dot 585 streptavidin conjugate (Qdot), rhodamine-phalloidin (Rh-ph), biotin-XX-phalloidin, and phalloidin were obtained from Life Technologies (Grand Island, NY). Glucose oxidase was purchased from MP Biomedicals (Santa Ana, CA). Biotin free bovine serum albumin (BSA, cat # A3059) and catalase were from Sigma-Aldrich (St. Louis, MO). Other chemicals were purchased from Sigma-Aldrich or Affymetrix (Cleveland, OH).
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2

Fluorescent Protein Labeling Techniques

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Quantum dot 585 streptavidin conjugate (Qdot), rhodamine-phalloidin, biotin-XX-phalloidin, phalloidin, and 10% Tris-Glycine Mini Gels were obtained from Life Technologies (Grand Island, NY). Glucose oxidase was purchased from MP Biomedicals (Santa Ana, CA). Biotin free bovine serum albumin (BSA, cat # A3059) and catalase were from Sigma-Aldrich (St. Louis, MO). Other chemicals were purchased from Sigma-Aldrich or Affymetrix (Cleveland, OH). Protein concentrations were measured using the Bradford assay (Bio-Rad, Hercules, CA).
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