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Uv 1601 pc spectrophotometer

Manufactured by Shimadzu
Sourced in Japan

The UV-1601 PC spectrophotometer is a compact, general-purpose UV-visible spectrophotometer designed for routine analysis. It features a wavelength range of 190 to 1100 nm and can perform absorbance, transmittance, and concentration measurements. The instrument includes a Windows-based software interface for data acquisition and analysis.

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45 protocols using uv 1601 pc spectrophotometer

1

Absorbance Spectral Measurement at 20°C

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Absorbance
spectra were recorded using a Shimadzu UV-1601PC spectrophotometer.
The measurements were carried out at 20 °C.
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2

Antioxidant Activity of Fruit Pomaces and Experimental Diets

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The antioxidant activity of fruit pomaces was measured directly in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay according to the method of Hatano [26 ], and the antioxidant activity of experimental diets was determined in accordance with the method described by Zielińska et al. [27 (link)]. The antioxidant activity of diets was also measured in the ABTS•+ assay in line with the method proposed by Re et al. [28 ] and the photochemiluminescence assay against superoxide anion radical ( O2-) according to the method of Popov and Lewin [29 ]. Measurements were carried out in a temperature-controlled UV-160 1PC spectrophotometer with a CPS-Controller (Shimadzu, Tokyo, Japan), and the results were expressed in μmol Trolox/g of sample. The photochemiluminescence assay was used to determine total antioxidant capacity as the sum of the antioxidant potentials of hydrophilic (ACW) and lipophilic (ACL) fractions. The ACL fraction was extracted from samples with 80% methanol, followed by a mixture of methanol and hexane (4:1, v/v). Measurements were performed in a Photochem® apparatus with ACW and ACL analytical kits supplied by Analytik Jena (Leipzig, Germany).
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3

Spectroscopic characterization of compounds

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We measured the UV spectra using a UV-1601 PC spectrophotometer (Shimadzu, Kyoto, Japan). The CD spectra were obtained on a Chirascan-plus Quick Start CD Spectrometer (Applied Photophysics Limited, Leatherhead, UK) (acetonitrile, 20 °C). We recorded the 1H, 13C, and two-dimensional NMR spectra in acetone-d6 using a Bruker AVANCE III DRX-700 NMR instrument (Bruker, Karlsruhe, Germany).
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4

Multimodal Characterization of S,N-CQDs

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UV-1601 PC spectrophotometer was used to carry out UV spectrophotometric measurements (Shimadzu, Kyoto, Japan) using a 1 cm quartz cell. Agilent Technologies' Cary Eclipse fluorescence spectrophotometer was used for fluorescence measurements (Santa Clara, USA). The slit width was adjusted to 5 nm and the instrument was set to 750 V mode. Thermo-Fisher Scientific Nicolet—iS10 FT-IR spectrometer was used to obtain the FT-IR spectra (Waltham, MA, USA). It had a 4000–1000 cm−1 deuterated triglycine sulfate (DTGS) detector and a Ge/KBr beam splitter. The measurements were taken in 32 scans with a resolution of 4 cm−1. A JEM-2100 high-resolution transmission electron microscope (HRTEM) (JEOL, Tokyo) working at 200 kV was used to investigate morphology of S,N-CQDs. pH-meter (Consort, NVP- 901, Belgium) was also used.
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5

Insulin Loading Capacity of Microparticles

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Lyophilized samples were redispersed in 1 ml DI, then centrifuged for 8 min at 13 680 rcf/12 000 rpm at 4 °C using Hermle z111 cooling centrifuge, (Hermle, Germany). The supernatant was removed and the MPs were washed with 100 µl DI to remove any excess peptide on the MP surface then the loaded insulin was extracted by adding a mixture of 1 ml 0.05 M HCl/0.4 ml acetonitrile according to [3] (link). The extracted insulin was determined using a Shimadzu UV-1601 PC spectrophotometer (Shimadzu, Japan)
The loading capacity (LC%) was calculated according to the following equation:
LC%=(insulininMPswtoftheparticles)×100
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6

Spectroscopic Characterization of Compounds

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UV spectra were recorded on a Shimadzu UV-1601PC spectrophotometer in spectroscopic grade MeOH. IR spectra were recorded on a Shimadzu IR-460 spectrometer in pressed KBr disks. NMR spectra were recorded on Bruker AMX 400, operating at 400 MHz for proton and 100 MHz for carbon in spectroscopic grade CDCl3, DMSO-d6, and CD3OD. EI-MS (70 eV) were recorded on Agilent 7890A/5975C. TLC was carried out on precoated silica gel 60 F 254 aluminum sheets (Merck, Rahway, NJ, USA). Whatman chromatography paper (Grade 1 CHR) was obtained from GE Healthcare Life Sciences (Shanghai, China) and used with further adjustment of size. All chromatographic solvents were of reagent grade (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). For column chromatography, normal phase silica gel 60 (70–230 mesh, Merck), Sephadex LH-20 (25–100 μm, Sigma-Aldrich, St. Louis, MO, USA), and polyamide (particle size 50–160 μm, Sigma-Aldrich) were used.
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7

Comprehensive Spectroscopic Analysis of Compounds

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Optical rotation was measured using a JASCO DIP-180 digital spectropolarimeter. IR spectra were recorded using a Nicolet 510P FT-IR spectrometer. UV spectra were measured in MeOH using a Shimadzu UV-1601PC spectrophotometer. The NMR spectra were recorded in CDCl3 at room temperature on a Varian Mercury plus 400 NMR spectrometer with residual solvent resonance as an internal reference. The 2D NMR spectra were recorded using the standard pulse sequences. For EI-MS and HR-EI-MS, Finnigan TSQ-700 and JEOL SX-102A spectrometers were used, respectively. TLC was performed on silica gel 60 F254 plates (Merck, Darmstadt, Germany). Column chromatography was performed on silica gel (230 400 mesh ASTM, Merck). HPLC was performed using a Hitachi L-7000 chromatograph with a Bischoff RI detector (Leonberg, Germany). A normal phase column (LiChrosorb Si 60, 7 μm, 250 × 10 mm, Merck) was used for isolation.
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8

Characterization of Biphenyl Wrinkled Silica Nanoparticles

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The biphenyl wrinkled silica nanoparticles were analyzed by transmission electron microscopy (TEM) using an analytical JEOL 2100 microscope (Tokyo, Japan) with an accelerating voltage of 200 kV. Scanning electron microscopy (SEM) images were obtained using a Zeiss-LEO model 1530 scanning electron microscope (Monument, CO, USA). Nitrogen gas adsorption analysis used a Quantachrome AS1 autosorb (Graz, Austria). Thermogravimetric analysis (TGA) analysis was conducted using a TA Instruments SDT Q600. PTI QuantaMasterTM 30 Fluorescence Spectrophotometer (Birmingham, NJ, USA) was used to measure the fluorescence of BPWS and DOX. Ultraviolet Visible (UV–Vis) spectroscopy of doxorubicin was carried out using a Shimadzu UV-1601PC spectrophotometer (Kyoto, Japan). The fluorescence microscopic images were obtained using an Olympus IX71 microscope (Tokyo, Japan) with a PhotonMax 512B CCD camera (Princeton Instrument).
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9

DPPH Radical Scavenging Assay Protocol

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The DPPH radical scavenging capacity was determined using the method of the Lee et al. (27 (link)) with slight modifications. The soyasaponin, ascorbic acid, α-tocopherol, or BHT was standardized to give a stock solution (25 mg/mL) and filtered through a 20 μm Whatman paper no 4. Aliquots (25 μL) were placed in a cuvette, and an ethanolic solution of DPPH (100 μM) was added to a final volume of 1 mL. The decrease in absorbance at 515 nm was determined continuously with data capturing at 30 s intervals using a UV-1601 PC spectrophotometer (Shimadzu Corporation, Kyoto, Japan). The degree of DPPH radical scavenging activity of the antioxidants was calculated as percentage of inhibition (% inhibition) using the following equation:
where Abscontrol is the absorbance at 0 min and Abssample is the absorbance of the sample at 5 min. An EC50 value was determined as the concentration that elicited a half-maximal response.
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10

Spectrophotometric Analysis of HOCl and 5HT

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Spectra were recorded using a UV-1601PC spectrophotometer (Shimadzu, Kyoto, Japan). Scans were performed in PBS at pH 7.4 and 20°C in a quartz cell. Concentrations of HOCl ranged from 50 to 200µM with 5HT at 50µM. The recordings were done over the wavelength range of 190–700nm for 150 m.
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