BrdU (Fisher Scientific, # BP 2508250) was added to medium (3µg/ml) for 4 hrs, and fixed in 4% paraformaldehyde (PFA). Cells were incubated with α-BrdU (BD Biosciences, San Jose, CA, #347580) (1:1000), and then Alexa Fluor 594-conjuated Affinipure F (ab’)2 fragment goat anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch, West Grove, PA, code:115–586-003). Coverslips were mounted with Vectashield with DAPI (Vector, Burlingame, CA, H-1500). Images were obtained from 10 representative areas on each coverslip using a Leica DM2500 microscope with a DFC 365FX digital camera and the Leica Application Suite-Advanced Fluorescence software package (Leica Microsystems).
Application suite advanced fluorescence software package
Manufactured by Leica
The Leica Application Suite-Advanced Fluorescence (LAS AF) is a software package designed for the analysis and processing of fluorescence microscopy data. The software provides a comprehensive suite of tools for image acquisition, processing, and analysis, catering to the needs of researchers and scientists working in the field of advanced fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Bromodeoxyuridine (brdu), by Thermo Fisher Scientific (2 mentions)
α brdu, by BD (2 mentions)
Vectashield with dapi, by Vector Laboratories (2 mentions)
Alexa fluor 594 conjuated affinipure f ab 2 fragment goat anti mouse igg h l secondary antibody, by Jackson ImmunoResearch (2 mentions)
Dfc365 fx digital camera, by Leica (2 mentions)
2 protocols using application suite advanced fluorescence software package
1
BrdU Incorporation and Visualization
2
BrdU Incorporation and Visualization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BrdU (Fisher Scientific, # BP 2508250) was added to medium (3µg/ml) for 4 hrs, and fixed in 4% paraformaldehyde (PFA). Cells were incubated with α-BrdU (BD Biosciences, San Jose, CA, #347580) (1:1000), and then Alexa Fluor 594-conjuated Affinipure F (ab’)2 fragment goat anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch, West Grove, PA, code:115–586-003). Coverslips were mounted with Vectashield with DAPI (Vector, Burlingame, CA, H-1500). Images were obtained from 10 representative areas on each coverslip using a Leica DM2500 microscope with a DFC 365FX digital camera and the Leica Application Suite-Advanced Fluorescence software package (Leica Microsystems).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!