Example 1
Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, Ill.), NovaBiochem (La Jolla, Calif.), or Anaspec (San Jose, Calif.). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, Calif.). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, Mo.). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, Mass.). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, Calif.).