Perfection v300 photo scanner

Manufactured by Epson

Sourced in Japan

The Epson Perfection V300 Photo Scanner is a flatbed scanner that can digitize photographs, documents, and other media at up to 4800 dpi resolution. It features a USB 2.0 interface for connectivity.

Automatically generated - may contain errors

Lab products found in correlation

Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Antibody for ezh2, by Cell Signaling Technology (1 mentions) Alphaeasefc software, by Bio-Techne (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Ly3214996, by Selleck Chemicals (1 mentions) Gdc 0623, by Selleck Chemicals (1 mentions) Ro5126766 ch5126766, by Selleck Chemicals (1 mentions) Sch772984, by Selleck Chemicals (1 mentions)

6 protocols using perfection v300 photo scanner

Panicle Surface Area Measurement

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Areas of spikelets and branches in a panicle were calculated to obtain the total area of a panicle. Specifically, the non-degraded green spikelets were firstly detached from the panicle 5 days after heading; then, these spikelets were scanned with an Epson Perfection V300 Photo scanner (Epson, Tokyo, Japan; Additional file 1: Figure S1a). The areas of spikelets in the scanned images were obtained by image processing using ImageJ (US National Institutes of Health, Bethesda, USA). The flag leaf area was obtained in the same way (Additional file 1: Figure S1c).
The spikelet-less panicles were then spread out, scanned with an Epson Perfection V300 Photo scanner as well (Epson, Tokyo, Japan; Additional file 1: Figure S1b). The projected area of branches was obtained through image processing using ImageJ (US National Institutes of Health, Bethesda, USA). Branches were assumed as cylinders, and single-sided surface area of all branches on a panicle S was then calculated as:

S = S_{bp} * π / 2 .

in which s_bp is the projected area of branches. Single-sided surface area of a whole panicle was calculated as a sum of the area of branches and the area of all spikelets.

Chang T.G., Song Q.F., Zhao H.L., Chang S., Xin C., Qu M, & Zhu X.G. (2020). An in situ approach to characterizing photosynthetic gas exchange of rice panicle. Plant Methods, 16, 92.

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Quantification of Myocardial Infarct Size

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At the end of reperfusion period, hearts were frozen at −80°C for 30 min. The frozen hearts were cut transversely into 5 pieces, each 1 mm thick, and stained with 1% triphenyl tetrazolium chloride (TTC) for 30 min in a 37°C water bath.[18 (link)] Then, the slices were fixed in 4% formaldehyde solution for 15 min. At last, each slice was photographed by Epson Perfection V300 Photo Scanner (Epson, Japan). The viable myocardium presented brick red, and infarct tissues appeared pale white. Infarct size and left ventricular area were measured using Image-J software (National Institutes of Health, USA), with the infarct size expressed as a percentage of the total left ventricular area (×100%).

Liu J.D., Chen H.J., Wang D.L., Wang H, & Deng Q. (2017). Pim-1 Kinase Regulating Dynamics Related Protein 1 Mediates Sevoflurane Postconditioning-induced Cardioprotection. Chinese Medical Journal, 130(3), 309-317.

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Western Blot Analysis of EZH2

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Cells were harvested in RIPA lysis buffer (Beyotime, China). Equal amounts of protein were separated by SDS-PAGE and transferred onto polyvinylidene fluoride membranes (Millipore, USA). The membranes were blocked in phosphate-buffered saline/Tween-20 containing 5% non-fat milk and incubated with antibody for EZH2 (Cell Signaling Technology, USA) or β-actin (Santa Cruz biotechnology). Then, the membranes were incubated with HRP-labeled IgG (KPL, USA) and detected using an Epson Perfection V300 Photo Scanner (Epson, Japan). Quantitative analysis was performed using AlphaEase FC software (Alpha Innotech, USA). Protein levels were normalized to β-actin.

Sun N.X., Ye C., Zhao Q., Zhang Q., Xu C., Wang S.B., Jin Z.J., Sun S.H., Wang F, & Li W. (2014). Long Noncoding RNA-EBIC Promotes Tumor Cell Invasion by Binding to EZH2 and Repressing E-Cadherin in Cervical Cancer. PLoS ONE, 9(7), e100340.

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Myocardial Infarct Size Quantification

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At the end of the reperfusion period, hearts were frozen at − 80°C for 30 min. The frozen hearts were cut transversely into 5 pieces, each 1 mm thick, and stained with 1% TTC for 30 min in a 37°C water bath. Then the slices were fixed in 4% formaldehyde solution for 15 min. At last, each slice was photographed by Epson Perfection V300 Photo scanner. The viable myocardium stained brick red, and infarct tissues appeared pale white. Infarct and LV area were measured using Image-J software, with the infarct size expressed as a percentage of the total LV area.

Liu J.D., Deng Q., Tian H.H., Pang Y.T, & Deng G.L. (2015). Wnt/Glycogen Synthase Kinase 3β/β-catenin Signaling Activation Mediated Sevoflurane Preconditioning-induced Cardioprotection. Chinese Medical Journal, 128(17), 2346-2353.

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Assessing Long-term Drug Resistance

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Long-term drug resistance was assessed by seeding in parallel 2.5 × 10⁵ and 5 × 10⁵ cells in 6-cm plates and 24 h after plating cells were treated with 1 μM of a particular inhibitor as single or double treatment. For the plates where the initial cell number was 2.5 × 10⁵, medium and inhibitor(s) were changed every 3 days for 14 days. Cells were fixed and stained with Crystal Violet solution, pictures were captured with EPSON PERFECTION V300 PHOTO scanner and images acquired with the paired software. taken. For the plates whose initial cell number was 5 × 10⁵, medium and inhibitor(s) were changed every 3 days until plates were fully confluent. At confluence plates were split 1:5 to one 6-cm-plate without inhibitor(s) (1/5), in order to test for possible drug addiction, a 10-cm-plate with inhibitor(s) (3/5) for expansion/freezing and one 6-cm-plate with inhibitor(s) (1/5) for future protein isolation. Days after resistance were counted from the splitting time until the plates reached confluence again. Inhibitors used were RO5126766 (CH5126766) (Selleckchem, Houston, TX, USA, S7170), GDC-0623 (Selleckchem, Houston, TX, USA, S7553), SCH772984 (Selleckchem, Houston, TX, USA, S7101), LY3214996 (Selleckchem, Houston, TX, USA, S8534). Final DMSO concentration was kept at 0.1% in control and inhibitor-treated cells for single treatments and 0.2% for double treatments.

Catalano A., Adlesic M., Kaltenbacher T., Klar R.F., Albers J., Seidel P., Brandt L.P., Hejhal T., Busenhart P., Röhner N., Zodel K., Fritsch K., Wild P.J., Duyster J., Fritsch R., Brummer T, & Frew I.J. (2021). Sensitivity and Resistance of Oncogenic RAS-Driven Tumors to Dual MEK and ERK Inhibition. Cancers, 13(8), 1852.

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Pseudomonas-Aspergillus Interaction Assay

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AF293 lawns were prepared and incubated at 25 °C for 12 h, followed by spot-inoculating 10 μl aliquots of early stationary phase PA14 cultures (DKN370, wild-type, phzM::TnM, or Δphz) onto the preformed fungal lawns. The co-cultures and axenic cultures of PA14 strains and AF293 were incubated at 25 °C for an additional 7 day. Plates of co-cultures and axenic control cultures were imaged daily using an EPSON Perfection V300 Photo Scanner at 600 dpi resolution.

Zheng H., Kim J., Liew M., Yan J.K., Herrera O., Bok J., Kelleher N.L., Keller N.P, & Wang Y. (2014). Redox metabolites signal polymicrobial biofilm development via the NapA oxidative stress cascade in Aspergillus. Current biology : CB, 25(1), 29-37.

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