Log In Sign up
The largest database of trusted experimental protocols

Hmgn1

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

HMGN1 is a nonhistone chromosomal protein that binds to nucleosomes and plays a role in regulating chromatin structure and gene expression. It functions as an architectural protein that interacts with DNA and histones to facilitate access to the underlying genetic information.

Automatically generated - may contain errors

Lab products found in correlation

Hmgn1, by Aviva Systems Biology (2 mentions) α tubulin, by Merck Group (2 mentions) Total h3, by Cell Signaling Technology (2 mentions) Cd24 pe cy7, by BD (2 mentions) Cd43 fitc, by BD (2 mentions) Cd45.1 pecy7, by Thermo Fisher Scientific (2 mentions) B220 pacific blue, by BD (2 mentions) Cd43 apc, by BD (2 mentions) Bp1 pe, by Thermo Fisher Scientific (2 mentions) Bp1 fitc, by Thermo Fisher Scientific (2 mentions) Cd45.2 apc, by Thermo Fisher Scientific (2 mentions) H3k27me3, by Cell Signaling Technology (2 mentions) H3k4me3, by Abcam (2 mentions) H3k27ac, by Abcam (2 mentions) P erk, by Cell Signaling Technology (1 mentions) Benchmark xt, by Roche (1 mentions) Hmgb1, by Abcam (1 mentions)

3 protocols using hmgn1

1

Western Blotting and Flow Cytometry Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting antibodies were HMGN1 (Aviva Systems Biology, #ARP38532_P050), HMGN1 (Abcam, #ab5212), mouse HMGN1 (affinity purified rabbit polyclonal)34 (link),35 (link), H3K27me3 (Cell Signaling Technologies, #9733, rabbit polyclonal), total H3 (Cell Signaling Technologies, #9715, rabbit polyclonal), and α-tubulin (Sigma, #T9026, mouse monoclonal). Flow cytometry antibodies were B220-Pacific Blue (BD Pharmingen, #558108, clone RA3-6B2), CD43-APC (BD, #560663, clone S7) or CD43-FITC (BD, #561856, clone S7), CD24-PE-Cy7 (BD, #560536, clone M1/69), BP1-PE (eBiosciences, 12-5891, clone 6C3) or BP1-FITC (eBiosciences, 11-5891, clone 6C3), CD45.1-PE-Cy7 (eBiosciences, 25-0453, clone A20), and CD45.2-APC (eBiosciences, 17-0454, clone 104). ChIP-seq antibodies were H3K27me3 (Cell Signaling Technologies, #9733), H3K4me3 (Abcam, #ab8580), and H3K27ac (Abcam, #ab4729).
Lane A.A., Chapuy B., Lin C.Y., Tivey T., Li H., Townsend E.C., van Bodegom D., Day T.A., Wu S.C., Liu H., Yoda A., Alexe G., Schinzel A.C., Sullivan T.J., Malinge S., Taylor J.E., Stegmaier K., Jaffe J.D., Bustin M., te Kronnie G., Izraeli S., Harris M., Stevenson K.E., Neuberg D., Silverman L.B., Sallan S.E., Bradner J.E., Hahn W.C., Crispino J.D., Pellman D, & Weinstock D.M. (2014). Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 lysine 27 trimethylation. Nature genetics, 46(6), 618-623.
+ Open protocol
+ Expand
2

Western Blotting and Flow Cytometry Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting antibodies were HMGN1 (Aviva Systems Biology, #ARP38532_P050), HMGN1 (Abcam, #ab5212), mouse HMGN1 (affinity purified rabbit polyclonal)34 (link),35 (link), H3K27me3 (Cell Signaling Technologies, #9733, rabbit polyclonal), total H3 (Cell Signaling Technologies, #9715, rabbit polyclonal), and α-tubulin (Sigma, #T9026, mouse monoclonal). Flow cytometry antibodies were B220-Pacific Blue (BD Pharmingen, #558108, clone RA3-6B2), CD43-APC (BD, #560663, clone S7) or CD43-FITC (BD, #561856, clone S7), CD24-PE-Cy7 (BD, #560536, clone M1/69), BP1-PE (eBiosciences, 12-5891, clone 6C3) or BP1-FITC (eBiosciences, 11-5891, clone 6C3), CD45.1-PE-Cy7 (eBiosciences, 25-0453, clone A20), and CD45.2-APC (eBiosciences, 17-0454, clone 104). ChIP-seq antibodies were H3K27me3 (Cell Signaling Technologies, #9733), H3K4me3 (Abcam, #ab8580), and H3K27ac (Abcam, #ab4729).
Lane A.A., Chapuy B., Lin C.Y., Tivey T., Li H., Townsend E.C., van Bodegom D., Day T.A., Wu S.C., Liu H., Yoda A., Alexe G., Schinzel A.C., Sullivan T.J., Malinge S., Taylor J.E., Stegmaier K., Jaffe J.D., Bustin M., te Kronnie G., Izraeli S., Harris M., Stevenson K.E., Neuberg D., Silverman L.B., Sallan S.E., Bradner J.E., Hahn W.C., Crispino J.D., Pellman D, & Weinstock D.M. (2014). Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 lysine 27 trimethylation. Nature genetics, 46(6), 618-623.
+ Open protocol
+ Expand
3

Immunohistochemical Profiling of Cellular Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed paraffin-embedded tissue samples were arrayed with a tissue-arraying instrument as previously described [35 (link)]. Each sample was arrayed in three 1-mm diameter cores to minimize tissue loss and overcome tumor heterogeneity. Tissue microarray sections were stained with an automatic immunohistochemical staining device (Benchmark XT; Ventana Medical Systems, Tucson, AZ). Antibodies to HMGB1 (1:200; Cat No. ab18256; Abcam, Cambridge, UK), HMGN1 (1:1000; Cat No. ab5212; Abcam), PERK (1:200; Cell Signaling Technology, Danvers, MA), p-eIF2α (1:200; Cat No. ab32157; Abcam), and XBP-1 (1:75; Cat No. ab37152; Abcam) were used. Protein expression was evaluated as a four-value intensity score (0, 1, 2, and 3). (Supplementary Figure S1 and S2) The percentage of nuclear and/or cytoplasmic expression was also evaluated. An ‘immunoreactive score’ was generated as the product of the intensity and the percentage of positive cells. The immunoreactive scores were dichotomized by the mean value of the expression of each protein.
Park I.A., Heo S.H., Song I.H., Kim Y.A., Park H.S., Bang W.S., Park S.Y., Jo J.H., Lee H.J, & Gong G. (2016). Endoplasmic reticulum stress induces secretion of high-mobility group proteins and is associated with tumor-infiltrating lymphocytes in triple-negative breast cancer. Oncotarget, 7(37), 59957-59964.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!