Adult Exaiptasia were paralyzed in anesthetic solution (15% MgCl2), rinsed in PBS, then embedded in Tissue-Tek O.C.T. Compound (Sakura Cat #4583) in cryomolds (Sakura Tissue-Tek® Cryomold®, Intermediate, Cat #4566) and flash frozen on dry ice and stored at −80°C. Cryostat sections (18–20μm) were adhered to Fisherbrand™ Superfrost™ Plus Microscope Slides (Fisher Scientific Cat #12–550-15) and flash frozen on dry ice and stored at −80°C until used for BaseScope. The BaseScope Duplex Detection Reagent Kit (Advanced Cell Diagnostics Cat #323800) and the manufacturer’s manual (BaseScope Duplex Detection Reagent User Manual, ACDBio) was followed to hybridize custom probes or positive control (Cat #700101) or negative control probes (Cat #700141) to targets in tissue cryosections and amplify signals. Samples were imaged on an Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Cat #BX41-PH-B) and images were acquired using the Olympus CellSens software and Olympus DP25 5MP Color Firewire Camera.
Dp25 5mp color firewire camera
Manufactured by Olympus
The DP25 5MP Color Firewire Camera is a compact, high-resolution digital camera designed for microscopy and imaging applications. It features a 5-megapixel sensor that captures detailed, color-accurate images. The camera connects to a computer via Firewire interface for seamless integration with imaging software.
Automatically generated - may contain errors
Lab products found in correlation
Bx41 phase contrast darkfield microscope, by Olympus (2 mentions)
Basescope duplex detection reagent kit, by Advanced Cell Diagnostics (2 mentions)
Fisherbrand superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions)
Superfrost plus microscope slide, by Thermo Fisher Scientific (1 mentions)
2 protocols using dp25 5mp color firewire camera
1
Cryosectioning and in situ mRNA Detection in Exaiptasia
2
Cryosectioning and BaseScope Analysis of Exaiptasia
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Adult Exaiptasia were paralyzed in anesthetic solution (15% MgCl2), rinsed in PBS, then embedded in Tissue-Tek O.C.T. Compound (Sakura Cat #4583) in cryomolds (Sakura Tissue-Tek Cryomold, Intermediate, Cat #4566) and flash frozen on dry ice and stored at –80 °C. Cryostat sections (18–20 µm) were adhered to Fisherbrand Superfrost Plus Microscope Slides (Fisher Scientific Cat #12-550-15) and flash frozen on dry ice and stored at –80 °C until used for BaseScope. The BaseScope Duplex Detection Reagent Kit (Advanced Cell Diagnostics Cat #323800) and the manufacturer’s manual (BaseScope Duplex Detection Reagent User Manual, ACDBio) was followed to hybridize custom probes or positive control (Cat #700101) or negative control probes (Cat #700141) to targets in tissue cryosections and amplify signals. Samples were imaged on an Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Cat #BX41-PH-B) and images were acquired using the Olympus CellSens software and Olympus DP25 5MP Color Firewire Camera.
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