A015 3

Manufactured by Nanjing Jiancheng

Sourced in China

The A015-3 is a laboratory equipment designed for analytical purposes. It features a compact and durable construction for standard laboratory use.

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Lab products found in correlation

A001 3, by Nanjing Jiancheng (2 mentions) M8650, by Solarbio (1 mentions) Microplate reader, by Bio-Rad (1 mentions) S0051, by Beyotime (1 mentions)

Spelling variants of this product

A015-3-1 (2 citations)

2 protocols using a015 3

Oxidative Stress and Mitochondrial Dysfunction Assessment

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ROS production was detected, based on the oxidation of 7-dichlorodihydrofluorescein diacetate (DCFH-DA) to 2,7-dichlorodihydrofluorescein, with a ROS detection kit (Boster, China) following manufacture’ instruction. DNA damage was observed via 8OHdG immunofluorescence staining. Mitochondrial membrane potential (MMP) in PMEVCs were detected by JC-1 staining (M8650, Solarbio, Beijing, China) according to the manufacturer's instructions. Culture medium supernatant were collected by centrifugation at 12,000×g for 5 min to measure antioxidant activity, including ferric ion reducing antioxidant power (FRAP) (A015-3, Nanjing Jiancheng Bioengineering Institute, China), GSH (A005, Nanjing Jiancheng Bioengineering Institute), or SOD (A001-3, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer's instructions. Each determination was made the average of at least three independent experiments.

Shen K., Wang X., Wang Y., Jia Y., Zhang Y., Wang K., Luo L., Cai W., Li J., Li S., Du Y., Zhang L., Zhang H., Chen Y., Xu C., Zhang J., Wang R., Yang X., Wang Y, & Hu D. (2023). miR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury. Redox Biology, 62, 102655.

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Antioxidant Activity of MSC-EVs in Hippocampal Neurons

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The antioxidant activity of MSC-EVs on H₂O₂-stimulated primary cultures of hippocampal neurons was tested according to the procedures described in our recent study 21 (link). The cell supernatant in each group (n = 6~9 samples per group) was collected by centrifugation at 12000 g for 5 min, and the samples and reaction reagents, including those from the ferric ion reducing antioxidant power (FRAP) (A015-3, Nanjing Jiancheng Bioengineering Institute, China), catalase (CAT) (S0051, Beyotime, China), glutathione peroxidase (GSH-PX) (A005, Nanjing Jiancheng Bioengineering Institute, China) and superoxide dismutase (SOD) (A001-3, Nanjing Jiancheng Bioengineering Institute, China) kits, were added to 96-well flat-bottom plates. The antioxidant effect or enzyme activity was detected using a microplate reader (Bio-Rad Laboratories Inc., Hercules, CA, USA) per the manufacturer's instructions. Each test was replicated 3 times.

Luo Q., Xian P., Wang T., Wu S., Sun T., Wang W., Wang B., Yang H., Yang Y., Wang H., Liu W, & Long Q. (2021). Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage. Theranostics, 11(12), 5986-6005.

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