Tcc 3000rs column

The UPLC system (Thermo Fisher Scientific) included an Ultimate 3000 RSLC system
with binary pumps, a WPS-3000TRS autosampler, and a TCC-3000RS column oven. The
chromatographic separation was performed using an Ultimate XB-C₁₈column (2.1 mm × 50 mm, 1.8 μm; Welch Materials, Shanghai, China). The binary
mobile phase system consisted of 0.1% formic acid in water (A) and 0.1% formic
acid in acetonitrile (B). The gradient program was as follows: 0 to 3.5 minutes,
5% to 95% B; 3.5 to 4.5 minutes, 95% B; 4.5 to 5.0 minutes, 95% to 5% B; and 5.0
to 6.0 minutes, 5% B. The flow rate was 0.4 mL·minute⁻¹. The column
temperature was 45°C.
The MS analysis was performed on a TSQ Quantum Access MAX (Thermo Fisher
Scientific) equipped with electrospray ionization. The compounds were ionized in
the positive and negative ion modes. The optimized parameters of the MS analysis
were set as follows: spray voltage, 4000 V (positive and negative); capillary
temperature, 350°C; sheath gas (nitrogen) pressure, 40 arb; and aux gas
(nitrogen) pressure, 15 arb. Argon was used as the collision gas. Quantification
was performed using the selected reaction monitoring (SRM) mode. The SRM
transitions and conditions for measurement of the compounds are summarized in
Table 1. Data
were acquired using ThermoXcalibur software (version 3.0, Thermo Fisher
Scientific).