Biotinylated rat igg2b

O-(2-Aminoethyl)-O’-[2-(biotinylamino)ethyl]octaethylene glycol (Biotin-PEG-NH₂, ≥ 95 %, M_w= 3400), 1,6-hexanediol diacrylate (HDD, 99 %), 3-amino-1-propanol (AP, 99 %), TPCA-1 (≥ 95 %, [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide) and lipopolysaccharide (LPS, Escherichia coli 0111:B4) were purchased from Sigma-Aldrich (St. Louis, MO). Cyanine 5 NHS ester (Cy5-NHS) was obtained from Lumiprobe Co., USA. Fluorescein isothiocyanate (FITC) was purchased from Alfa Aesar. NeutrAvidin™ biotin binding protein was purchased from Thermo Fisher Scientific. All chemical reagents were used as received. Biotinylated rat IgG2b and biotinylated anti- ICAM-1 antibodies (Abs) were purchased from Biolegend (San Diego, CA). Pierce™ BCA protein assay kit was purchased from Thermo Fisher Scientific. All cell lines, biological reagents and other cell culture medium were used as received following our previous protocols.^{13 (link)}