Hemin, zinc protoporphyrin (ZnPP-IX), L-nitroarginine methyl ester (L-NAME), sodium nitroprusside (SNP), aminooxyacetic acid (AOAA), ethylenediamine tetra-acetic acid solution (EDTA), zinc acetate, ferric chloride, borate buffer (pH 11.0) and N, N-dimethyl-p-phenylenediamine sulfate were all purchased from Sigma–Aldrich, St. Louis, MO. All reagents were freshly prepared in distilled water, except N, N-dimethyl-p-phenylenediamine which was prepared in 7.2 M hydrochloric acid, immediately before use in the series of experiments.
N n dimethyl p phenylenediamine sulfate
Manufactured by Merck Group
Sourced in United States
N,N-dimethyl-p-phenylenediamine sulfate is a chemical compound commonly used as a reagent in analytical and clinical laboratories. It is a salt composed of N,N-dimethyl-p-phenylenediamine and sulfuric acid. The compound is a colorless to pale yellow crystalline solid.
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Lab products found in correlation
Ferric chloride, by Merck Group (1 mentions)
Recombinant human il 1β, by Thermo Fisher Scientific (1 mentions)
Icam 1, by Cell Signaling Technology (1 mentions)
Keap1, by Santa Cruz Biotechnology (1 mentions)
Interleukin 6 (il 6), by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Zinc acetate, by Merck Group (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
2 3 5 triphenyltetrazolium chloride ttc, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphate sodium salt, by Avanti Polar Lipids (1 mentions)
4 protocols using n n dimethyl p phenylenediamine sulfate
1
Biochemical Reagents for Experimental Protocols
2
Recombinant IL-1β Inflammatory Pathway Study
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Recombinant human IL-1β was purchased from Peprotech (Rocky Hill, NJ, USA). Freund's adjuvant (FA, heat-inactivated Mycobacterium tuberculosis) was obtained from Chondrex. 3-(4,5-Dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT), DL-propargylglycine (PAG), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), 2-aminobicyclo-2(2,2,1)-heptane-2-caboxylic acid (BCH), and N,N-dimethyl-p-phenylenediamine sulfate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies were obtained from the following commercial sources: MMP-9, IL-6, and ICAM-1 were purchased from Cell Signaling Biotechnology (Danvers, MA, USA); Keap-1, HO-1, CSE, CBS, Nrf-2, SOD1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); and antibody against Lamin A/C was obtained from Epitomics (Burlingame, CA, USA). SPRC was synthesized in our laboratory and purified by recrystallization from ethanol–water mixture (99%).
3
Rat Cardiac Ischemia-Reperfusion Injury Model
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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphate sodium salt (DPPA), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-5000] ammonium salt (DPPE-PEG5000) were purchased from Avanti Polar-lipids (Alabaster, AL, USA). H2S and C3F8 were obtained from Foshan Kodi Gas Chemiacal Industry Co., Ltd (Foshan, China). Zinc acetate, N, N- dimethyl-pphenylenediamine sulfate, Na2S, 2,3,5-triphenyltetrazolium chloride (TTC) were purchased from Sigma-Aldrich Co (St Louis, MO, USA). In Situ Cell Death Detection Kit was purchased from Roche Applied Science (Mannheim, Germany). Propylene glycol, glycerol and ferric trichloride were obtained from Guangdong Guanghua Sci-Tech Co. Ltd (Guangzhou, China). All reagents used in the present study were of analytical grade.
A total of 99 Sprague-Dawley rats (weight 250 to 300 g) were supplied by Southern Medical University (Guangzhou, China). All animal experiments were approved by the Animal Research Committee of the Southern Medical University and were carried out in accordance with the Guide for the Care and Use of Laboratory Animals published by US National Institutes Health (NIH Publication No. 85-23, updated 2011).
A total of 99 Sprague-Dawley rats (weight 250 to 300 g) were supplied by Southern Medical University (Guangzhou, China). All animal experiments were approved by the Animal Research Committee of the Southern Medical University and were carried out in accordance with the Guide for the Care and Use of Laboratory Animals published by US National Institutes Health (NIH Publication No. 85-23, updated 2011).
4
Determination of Sulfide Concentration
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A 100 mgS L -1 standard sulfide solution was prepared by dissolving sodium sulfide (Na2S•9H2O, 1.0 g; Wako Pure Chemicals, analytical grade) in deoxygenated water (100 mL). The prepared solution was standardized iodometrically.
A sulfide coloring reagent was prepared by dissolving N,N-dimethyl-p-phenylenediamine sulfate (0.4 g; Sigma-Aldrich, technical grade) and iron(III) chloride (FeCl3•6H2O, 0.6 g; Wako Pure Chemicals, analytical grade) in 6 M HCl (100 mL; Wako Pure Chemicals, analytical grade).
Deoxygenated water was prepared by thorough treatment with N2 gas (>99.99%) to remove dissolved oxygen. A Shimadzu UV-1600 spectrophotometer with a 10-mm semi-micro glass cell (GL Sciences, 4 mm inside width) was used to determine sulfide concentration.
A sulfide coloring reagent was prepared by dissolving N,N-dimethyl-p-phenylenediamine sulfate (0.4 g; Sigma-Aldrich, technical grade) and iron(III) chloride (FeCl3•6H2O, 0.6 g; Wako Pure Chemicals, analytical grade) in 6 M HCl (100 mL; Wako Pure Chemicals, analytical grade).
Deoxygenated water was prepared by thorough treatment with N2 gas (>99.99%) to remove dissolved oxygen. A Shimadzu UV-1600 spectrophotometer with a 10-mm semi-micro glass cell (GL Sciences, 4 mm inside width) was used to determine sulfide concentration.
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