Nr8383

The biological characterization of the particles was performed with the cell line NR8383 (rat alveolar macrophages, LGC Standards GmbH, Wesel, Germany). The cells were cultivated with Ham's F-12 medium containing 15% fetal calf serum (FCS, GIBCO, Invitrogen, Karlsruhe, Germany) in 175 cm² cell culture flasks (BD Falcon, Becton Dickinson GmbH, Heidelberg, Germany) at standard cell culture conditions (humidified atmosphere, 37 °C, 5% CO₂). NR8383 cells were partly adherent and partly non-adherent. The ratio between adherent and non-adherent cells was about 1:1. For cell experiments, adherent cells were detached from the cell culture flasks with a TPP cell scraper (TPP Techno Plastic Products AG, Trasadingen, Switzerland), subsequently combined with non-adherent cells and seeded into 24-well cell culture plates (BD Falcon) at a concentration of 2.4 × 10⁵ cells cm⁻².

To study the biological activity of the TiO₂ particles, the rat alveolar macrophage cell line NR8383 (LGC Standards GmbH, Wesel, Germany) was used. Cultivation of cells was carried out in Ham’s F-12 medium with additional 15% fetal calf serum (FCS, GIBCO, Invitrogen, Karlsruhe, Germany) at standard cell culture conditions (humidified atmosphere, 37 °C, 5% CO₂) in 175 cm² cell culture flasks (BD Falcon, Becton Dickinson GmbH, Heidelberg, Germany). The partly adherent NR8383 cells (1:1 ratio of adherent:non-adherent cells) were scraped from cell culture flasks with a cell scraper (TPP Techno Plastic Products AG, Trasadingen, Switzerland) and mixed with the non-adherent cell portion before seeding 2.4 × 10⁵ cells cm⁻² in 24-well cell culture plates (BD Falcon; Fisher Scientific).

The biological effect of the particles was studied with the cell line NR8383 (rat alveolar macrophages, LGC Standards GmbH, Wesel, Germany). The cells were cultivated in Ham’s F12 medium containing 15% fetal calf serum (FCS, GIBCO, Invitrogen, Karlsruhe, Germany) in 175 cm² cell culture flasks (BD Falcon, Becton Dickinson GmbH, Heidelberg, Germany) at standard cell culture conditions (humidified atmosphere, 37 °C, 5% CO₂). The NR8383 cells were only partly adherent. The ratio between adherent and non-adherent cells was about 1:1. For cell experiments, adherent cells were detached from the cell culture flasks with a TPP cell scraper (TPP Techno Plastic Products AG, Trasadingen, Switzerland), subsequently combined with non-adherent cells, and seeded into 24-well cell culture plates (BD Falcon) at a concentration of 2.4 × 10⁵ cells cm⁻².