The remaining blood sample in the EDTA test tube was used to determine Hgb concentration, RBC, total WBC, and PLT counts using an automated hematology analyzer UniCel DxH 800 Coulter (Beckman Coulter, Inc, Miami, FL), following the protocol of the manufacturer. Major blood cells such as Hgb, RBC, total WBC count, and PLT were recorded. To define anemia in the patients, hemoglobin values of <12 g/dl for women and <13g/dl for men were used. Anemia was further classified as mild (Hgb 10.0–10.9 g/dl), moderate (Hgb 7–9.9 g/dl), and severe (Hgb <7g/dl).27 (link),28 A WBC count of >10,000 cells/μL was classified as leukocytosis, while leucopenia was defined as a WBC count <4000 cells/μL.29 (link) A PLT count <150, 000 cells/µL was used to define thrombocytopenia. It was further classified as mild (PLT between 100,000 and 150,000 cells/µL), moderate (PLT between 50,000 and 100,000 cells/µL), severe (PLT <50, 000 cells/µL).30 (link),31 (link)
Unicel dxh 800 coulter
Manufactured by Beckman Coulter
Sourced in United States
The UniCel® DxH 800 Coulter is a compact, automated hematology analyzer designed for clinical laboratories. It performs complete blood count (CBC) and white blood cell differential analysis. The instrument utilizes Beckman Coulter's proprietary Coulter Principle to provide accurate and reliable results.
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Lab products found in correlation
3 protocols using unicel dxh 800 coulter
1
Automated Hematology Analysis Protocol
2
Blood Cell Analysis Protocol
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At 24 h after the last administration, 500 μl of blood by heart puncture was put into a test tube containing ethylenediaminetetraacetic acid (EDTA; Lot No. WB07014, Well Biological Science Co., Ltd, Hunan Province, China), and the numbers of red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs) as well as the hemoglobin (Hb) content were detected by automatic blood cell analyzer (UniCel® DxH 800 Coulter, Beckman Coulter, Co., Ltd., USA).
3
Measuring Immune Markers in Samples
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The white blood cell (WBC) and neutrophil (NEUT) levels were measured by UniCel® DxH 800 Coulter® (Beckman Coulter, Inc., United States). The procalcitonin (PCT) level was measured by a double antibody sandwich immunochemiluminescence method (VIDAS 30, Shanghai Fengyue Trading Co., Ltd., China), and the interleukin-6 (IL-6) (ab178013, Abcam, United States) level was measured by ELISA. All experiments were repeated three times.
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