Analytical-grade standard of Chlorpyrifos (>99% purity) was purchased from Sigma-Aldrich, (Steinheim, Germany). The physico-chemical properties of Chlorpyrifos are tabulated in Supplementary Table 1. Pesticide stock solutions (1000 mg −1L) were prepared in HPLC grade methanol (Merck) and were stored in dark at −20 °C. LC grade acetonitrile, methanol, spectroscopic grade anhydrous magnesium sulfate, zinc chloride and cadmium chloride were purchased from Sigma–Aldrich. Deionized water for current experiment was prepared with a Milli-Q system from Millipore-Waters Co. (Bedford, MA).
Lc grade acetonitrile
Manufactured by Merck Group
Sourced in Germany, United States
LC-grade acetonitrile is a high-purity solvent used in liquid chromatography (LC) applications. It serves as a mobile phase component for HPLC and UHPLC separations, providing efficient analyte elution and separation. The product meets stringent quality standards to ensure reliable performance in analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (14 mentions)
Formic acid, by Merck Group (9 mentions)
Direct q3 purification system, by Merck Group (3 mentions)
Milli q water purification system, by Merck Group (3 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Podophyllotoxin, by Merck Group (2 mentions)
Milli q system, by Merck Group (2 mentions)
Ranitidine d 6, by Cerilliant (2 mentions)
Fluoxetine d 5, by Cerilliant (2 mentions)
Midazolam, by Merck Group (2 mentions)
Isopropanol, by Merck Group (2 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Zinc chloride, by Merck Group (1 mentions)
Cadmium chloride, by Merck Group (1 mentions)
Chlorpyrifos, by Merck Group (1 mentions)
Ferulic, by Merck Group (1 mentions)
Gallic, by Merck Group (1 mentions)
Vanillic, by Merck Group (1 mentions)
P coumaric, by Merck Group (1 mentions)
31 protocols using lc grade acetonitrile
1
Chlorpyrifos Analytical Protocol
2
Quantifying Polyphenol Standards Analysis
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Standards of caffeic, gallic, ferulic, isoferulic, protocatechuic, 4-OH-benzoic, vanillic, p-coumaric, salicylic, and LC grade acetonitrile were purchased from Sigma–Aldrich Fine Chemicals (St. Louis, MO, USA). Gentisic and sinapic acids were from ChromaDex (Irvine, CA, USA). The LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Ethanol and methanol (reagent grade purity), used for the preparation of extracts, Folin-Ciocalteu reagents, and all analytical grade reagents were obtained from Avantor Performance Materials (Center Valley, PA, USA).
3
Berberis vulgaris Chemical Analysis
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The plant material of the Berberis vulgaris L. barberry stem, leaves, and fruits was obtained from the Maria Curie-Skłodowska University Botanical Garden in Lublin in September 2020 (Voucher specimen: AO2020091). The raw material was separated and dried at room temperature in the shade with ventilation. The raw material was weighed and ground in an electric mill and portioned, vacuum-packed, and stored in a closed package at −30 °C until the start of the tests. DPPH• (2,2-diphenyl-1-picrylhydrazyl), Trolox, gallic acid, 3-caffeoylquinic acid, protocatechuic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid, caffeic acid, catechin, luteolin, eriodictyol-7-glucopyranoside, quercetin, syringaresinol-di-O-glucoside, rutin, hyperoside, luteoloside, isoquercetin, narcissoside, isorhamnetin-3-glucoside, quercitrin naringenin 7-glucoside, afzelin, LC grade acetonitrile, Trolox, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), Folin–Ciocalteu reagent, and 2,2′-azobis (2-methylpropionamide) dihydrochloride (AAPH) were purchased from Sigma–Aldrich (Stenheim, Germany); ascorbic acid was purchased from Stanlab (Poland); methanol and aluminium chloride hexahydrate of analytical grade were purchased from POCH (Gliwice, Poland).
4
Antioxidant Activity Determination Protocols
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LC grade acetonitrile, ascorbic acid, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), 2,2′-azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS•+), Folin–Ciocalteu reagent, nitrobluetetrazolium (NBT), xanthine, xanthine oxidase, and 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was obtained from Gibco (Carlsbad, CA, USA). Reference compounds of phenolic acids and flavonoids were purchased from ChromaDex (Irvine, CA, USA). Water and formic acid for LC analysis were from Merck (Darmstadt, Germany). Sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), and all the other chemicals were of analytical grade and were purchased from the Polish Chemical Reagent Company (POCH, Gliwice, Poland).
5
Cytotoxicity Assay with Podophyllotoxin
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Podophyllotoxin (standard compound, ≥98%), MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], LC grade acetonitrile, and RPMI 1640 medium were purchased from Sigma-Aldrich Fine Chemicals (Saint Louis, MO, USA), Fetal calf serum (FCS) for cell culture was purchased from Biochrom GmbH (Berlin, Germany), and DMSO was purchased from Fluka Chemie AG (Buchs, Switzerland). LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA).
6
Purification and Characterization of CGA Compounds
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CGA, CGA-ME, and CGA-LT were purified by us using D101 macroporous resin, Sephadex LH-20 and ODS column chromatography, and preparative HPLC from the aqueous extract of FE; the compounds were identified by UV, IR, MS, and NMR technologies [14 (link)]. Their purities above 95% were determined by UPLC analysis. LC-grade acetonitrile was purchased from Sigma-Aldrich (St. Louis, MO, USA). LC-grade water was produced by a Milli-Q water purification system (Millipore, Massachusetts, MA, USA). Formic acid was bought from Meridian Medical Technologies (MREDA, New York, NY, USA). Phosphoric acid, disodium hydrogen phosphate, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate were bought from DAMAO Chemical Reagent Factory (Tianjin, China).
The phosphate buffer solution at pH 2, 4, 6, and 8 was prepared according to the guidelines released by Chinese pharmacopoeia.
The phosphate buffer solution at pH 2, 4, 6, and 8 was prepared according to the guidelines released by Chinese pharmacopoeia.
7
Determination of Bisphenol Compounds
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Bisphenol A (BPA), bisphenol AP (BPAP), bisphenol AF (BPAF) and bisphenol P (BPP) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). Stock standard solutions of individual compounds (1000 mg L -1 ) and mix stock solutions containing Journal of Separation Science This article is protected by copyright. All rights reserved. 6 the four BPs (5 and 100 mg L -1 ) were prepared in LC grade methanol from Sigma-Aldrich and were stored in the dark at 4 ºC.
LC grade acetonitrile from Sigma-Aldrich and ultrapure water (resistivity of 18.2 MΩ cm at 25 °C) obtained from a Millipore Direct System Q5™ purification system from Ibérica S.A. (Madrid, Spain) were used to prepare the mobile phase of the LC system. This article is protected by copyright. All rights reserved.
LC grade acetonitrile from Sigma-Aldrich and ultrapure water (resistivity of 18.2 MΩ cm at 25 °C) obtained from a Millipore Direct System Q5™ purification system from Ibérica S.A. (Madrid, Spain) were used to prepare the mobile phase of the LC system. This article is protected by copyright. All rights reserved.
8
Antioxidant Activity Evaluation Protocol
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LC-grade acetonitrile, ascorbic acid, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), 2,20-azino-bis-(3-ethyl-benzothiazole-6-sulfonic acid) (ABTS●+), indomethacin and disodium dihydrate (Na2EDTA*2H2O), were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was obtained from Gibco (Carlsbad, CA, USA). Reference compounds were purchased from ChromaDex (Irvine, CA, USA). Water and formic acid for LC analysis were from Merck (Darmstadt, Germany). All the other chemicals were of analytical grade and were purchased from the Polish Chemical Reagent Company (POCH, Gliwice, Poland).
9
Phytochemical Analysis of Plant Extracts
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Reference standards: caffeic acid (≥98%), quinic acid (≥98%), catechin (≥98%), apigenin (≥95%), and apigenin-7-glucoside were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rutin was purchased from PhytoLab (Vestenbergsgreuth, Germany), and chlorogenic acid was purchased from the HWI group (Rülzheim, Germany). All solvents used were analytical or HPLC grade. The water was distilled and purified using the Stakpure GmpH water system (Niederahr, Germany). Gallic acid and AlCl3 and Trolox were purchased from Acros Organics (Geel, Belgium), and Na2CO3 and NaNO2 were obtained from Honeywell (Charlotte, NC, USA). 2,2-diphenyl-1-picrylhydrazyl (DPPH) was from Alfa Aesar (Kandel, Germany). Folin–Ciocalteu reagent, H2SO4, HCl, and NaOH reagents were purchased from Fisher Scientific (Loughborough, UK). LC-grade acetonitrile, methanol, and formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), and water for LC analysis was purified using a Stakpure GmpH water purification system (Niederahr, Germany).
10
Sterol Quantification by Isotope Dilution Mass Spectrometry
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2-picolinic acid (PA) and 2-methyl-6-nitrobenzonic anhydride (MNBA) were purchased from Aladdin (Shanghai, China), whereas 4-dimethylaminopyridine (DMPA) and anhydrous pyridine were obtained from J&K Scientific (Shanghai, China). All sterol standards examined in this study were purchased from J&K Scientific (Shanghai, China), Sigma-Aldrich (St Louis, MO, USA), Aladdin (Shanghai, China) and TRC (Toronto, Canada) as appropriate. Cholesterol-d7, campesterol-d3, estrone-d2, dehydroepiandrosterone-d2, estradiol-d2, 24-hydroxyCholesterol-d7, and β-Sitosterol-d7 were purchased from Sigma-Aldrich (St Louis, MO, USA), CDN ISOTOPES (Quebec, Canada) and CIL (Tewksbury, MA, USA) as appropriate. LC-grade acetonitrile and tert-butyl methyl ether were purchased from Sigma-Aldrich (St Louis, MO, USA), and pure water was obtained from a Milli-Q system (Merck Millipore, Germany).
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