AGS and MKN-28 cells were purchased from the Procell Life Science&Technology Co.,Ltd. (Wuhan, China, Cat. no. CL-0022, Cat. no. CL-0291). All cells were identified by STR, and STR typing showed no cross contamination of human cells in the cell lines. No mycoplasma was detected in the two cells. Cells were cultured in RPMI 1640 (HyClone, USA, Cat. no. SH30809.01) containing 10% fetal bovine serum (FBS) (TransGen Biotech, China, Cat. no. FS301) and the culture dishes (Corning, USA, Cat. no. 150,464) were placed in an incubator containing 5% CO2 at a constant temperature of 37℃. After the cells were overgrown in the culture dishes, the cells were digested and subcultured with 0.25% trypsin digestion solution (Beyotime Biotechnology, China, Cat. no. C0201).
Mkn 28
Manufactured by Procell
Sourced in China
The MKN-28 is a laboratory equipment designed for performing various scientific experiments and analyses. It features a digital display and control panel for precise temperature regulation and monitoring. The core function of the MKN-28 is to provide a controlled environment for conducting experiments or sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
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Hgc 27, by Procell (3 mentions)
Rpmi 1640, by Procell (2 mentions)
Ham s f 12, by Procell (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Kato 3, by Procell (2 mentions)
Trypsin digestion solution, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nci n87, by Procell (1 mentions)
Snu 1, by Procell (1 mentions)
Iscove modified dulbecco media (imdm), by Procell (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
7 protocols using mkn 28
1
Cell Culture Validation and Maintenance
2
Gastric Cell Line Culture Protocol
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Gastric cancer cell lines SGC-7901, MKN-28, BGC-823 and immortalized gastric mucosal epithelial cell line GES-1 purchased by the Procell Life Science &Technology (Procell, China) were cultured in RPMI-1640 medium (Hyclon, USA) with 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin at 37°C, a humidified 5% CO2 atmosphere.
3
Characterization of Gastric Cancer Tissue Samples
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Seven fresh frozen cancer tissues and corresponding paracancerous tissue samples were randomly selected from GC patients who underwent radical GC resection in the HMU Cancer Hospital from January 2019 to April 2019. The selected samples had complete clinicopathological data, including gender, age, degree of differentiation, tumor node metastasis (TNM) stage, primary tumor size, and lymph node metastasis. The Institutional Review Committee of Harbin Medical University Cancer Hospital approved this study. All samples were collected with the patient's written informed consent. The gastric epithelial cell line GES‐1 and GC cell lines AGS, BGC‐823, HGC‐27, and MKN‐28 were provided by Procell Life Science & Technology Co. Ltd. (Wuhan, PR China). AGS cells were cultured in Ham's F‐12 (Procell, PR China) and the other cells in RPMI‐1640 (Procell, Wuhan, PR China). All media were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution. All cells were incubated at 37 °C and 5% CO2.
4
Gastric Cancer Cell Line Cultures
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The gastric cancer cell lines (AGS, NCI-N87, SNU1, KATOIII and MKN28) and human gastric mucosa cell line (GES1) were obtained from Procell life science and Technology Co., Ltd (Wuhan, China). All the cell lines were cultured in RPMI-1640 medium (GIBCO, Los Angeles, CA, United States) with 10% fetal bovine serum (FBS, Gibco) and at 37°C in a 95% air, 5% CO2 humidified incubator.
5
Gastric Cell Line Culture Protocol
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The gastric epithelial cell line GES-1 and GC cell lines (AGS, BGC-823, HGC-27, MKN-28 and KATO III) were provided by Procell Life Science & Technology Co., Ltd. (Wuhan, China). AGS cells were cultured in Ham’s F-12 (Procell, CN), KATO III cells were cultured in IMDM (Procell, CN), and other cells were cultured in RPMI-1640 (Procell, CN). All culture media were supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution. All cells were cultured at 37 °C with 5% CO2.
6
Culturing Gastric Cancer Cell Lines
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Gastric cancer cell lines, BGC-823, SGC7901, AGS, MKN28, and HGC-27, and normal human gastric epithelial cells, GES-1, were obtained from Procell Life Science&Technology Co,.Ltd. (Wuhan, China) (http://www.procell.com.cn/ ). The cells other than AGS were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Procell Life Science & Technology Co,. Ltd.) and 100 U/mL penicillin streptomycin solution. AGS cells were cultured in F12 medium containing 10% FBS and 100 U/mL penicillin streptomycin solution. All cells were maintained at 37 °C in a saturated humidity atmosphere containing 95% air and 5% CO2. Cells were passaged when cell density reached 90%. After that, the medium was discarded and 1 mL sterile PBS was used to wash the Petri dish, and the cells were trypsinized and resuspended prior to cell passage [26 (link)].
7
Modulation of Gastric Cancer Cells
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The GES-1, MKN-28, SGC-7901, AGS, and BGC-823 cells were procured form Procell Life Science&Technology Co., Ltd (Wuhan, China). All the cells (1 × 104) were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with penicillin-streptomycin (1%) and fetal bovine serum (FBS) (10%), at 37°C in an incubator with 5% CO2. Replacement of culture substrate was performed every other day. The transfection was performed when the cells reached 90% confluency. The cells were transfected with lncRNA RPSAP52-shRNA#1, lncRNA RPSAP52-shRNA#2, miR-665 mimics, STAT3-shRNA#1, STAT3-shRNA#2, respectively, via using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the guidance of manufacturer.
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