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Ti inverted microscope

Manufactured by Lumencor
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Sourced in United States

The Ti inverted microscope is a high-quality optical instrument designed for scientific research and analysis. It features a robust and stable construction, providing a reliable platform for various microscopy techniques. The Ti inverted microscope allows for the observation and examination of samples from the bottom side, making it suitable for a wide range of applications.

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Lab products found in correlation

Spectra x light engine, by Lumencor (2 mentions) Scmos camera, by Oxford Instruments (2 mentions) Spectra x, by Lumencor (2 mentions) Poly l lysine coated slide, by Merck Group (1 mentions) Slow fade diamond mounting medium, by Thermo Fisher Scientific (1 mentions)

4 protocols using ti inverted microscope

1

Mounting Cells for Microscopy Imaging

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Cells were mounted onto poly-L-Lysine coated slides (Sigma) using SlowFadeTM Gold antifade reagent (Thermo Fisher Scientific, Waltham, MA, United States) and then imaged using a Nikon Ti inverted microscope fitted with a Lumencor Spectra X light engine. Images were obtained with a 100x PlanApo (1.4 NA) oil objective 1.518 RI oil, and an Andora Zyla sCMOS camera was used for detection.
Sutton J.A., Cooke M., Tinajero-Trejo M., Wacnik K., Salamaga B., Portman-Ross C., Lund V.A., Hobbs J.K, & Foster S.J. (2023). The roles of GpsB and DivIVA in Staphylococcus aureus growth and division. Frontiers in Microbiology, 14, 1241249.
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2

Fluorescence Imaging of Fixed Cells

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Fixed cells were dried onto a poly l-lysine-coated slide, mounted in PBS, and imaged on a Nikon Ti inverted microscope fitted with a Lumencor Spectra X light engine. Images were taken using a 100× PlanApo (1.4 NA) oil objective using 1.518 RI oil and detected by an Andor Zyla sCMOS camera.
Wacnik K., Rao V.A., Chen X., Lafage L., Pazos M., Booth S., Vollmer W., Hobbs J.K., Lewis R.J, & Foster S.J. (2022). Penicillin-Binding Protein 1 (PBP1) of Staphylococcus aureus Has Multiple Essential Functions in Cell Division. mBio, 13(4), e00669-22.
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3

Fluorescence Imaging of Fixed Cells

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Fixed cells were mounted on poly-l-Lysine coated slides and imaged on a Nikon Ti Inverted microscope fitted with a Lumencor Spectra X light engine. Images were taken using a 100x PlanApo (1.4 NA) oil objective using 1.518 RI oil and detected by an Andor Zyla sCMOS camera.
Lund V.A., Wacnik K., Turner R.D., Cotterell B.E., Walther C.G., Fenn S.J., Grein F., Wollman A.J., Leake M.C., Olivier N., Cadby A., Mesnage S., Jones S, & Foster S.J. (2018). Molecular coordination of Staphylococcus aureus cell division. eLife, 7, e32057.
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4

Fluorescent Cell Imaging on Poly-L-Lysine

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Fixed cells mounted onto poly-L-Lysine coated slides using Slow Fade Diamond mounting medium (Thermo Fisher, S36967) were imaged using a Nikon Ti inverted microscope fitted with a Lumencor Spectra X light engine. Images were obtained with a 100x PlanApo (1.4 NA) oil objective 1.518 RI oil and an Andora Zyla sCMOS camara was used for detection.
Tinajero-Trejo M., Carnell O., Kabli A.F., Pasquina-Lemonche L., Lafage L., Han A., Hobbs J.K, & Foster S.J. (2022). The Staphylococcus aureus cell division protein, DivIC, interacts with the cell wall and controls its biosynthesis. Communications Biology, 5, 1228.
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