Leukaemia inhibitory factor

Manufactured by Miltenyi Biotec

Sourced in China

Leukaemia inhibitory factor (LIF) is a cytokine that plays a role in the regulation of cell growth and differentiation. It functions as a glycoprotein that inhibits the differentiation of embryonic stem cells and maintains their pluripotency. LIF is commonly used in cell culture applications involving stem cells.

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Lab products found in correlation

Basic fibroblast growth factor (bfgf), by Sino Biological (1 mentions) Epidermal growth factor (egf), by Sino Biological (1 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions) Mem sodium pyruvate, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

2 protocols using leukaemia inhibitory factor

Cardiosphere-Derived Cell Culture

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Sorted cells were cultured in cardiosphere-growth medium (CGM) at 37 °C and 5% CO₂. The CGM was made up of CEM supplemented with 2% B27 (Thermo Fisher, Waltham, MA), 20 ng/mL basic fibroblast growth factor (bFGF) (Sino Biological, Beijing, China), 10 ng/mL epidermal growth factor (EGF) (Sino Biological, Beijing, China) and 10 ng/mL leukaemia inhibitory factor (LIF) (Miltenyi Biotec, Bergisch Gladbach, Germany).

Shen L., Fan G., Yang G., Yang Z, & Gui C. (2023). Paracrine effects of mir-210-3p on angiogenesis in hypoxia-treated c-kit-positive cardiac cells. Annals of Medicine, 55(2), 2237690.

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Mouse Embryonic Stem Cell Culture

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All cell lines are based on E14 mES cells, provided by E. Heard’s laboratory. Cells were cultured on gelatin-coated culture plates in Glasgow minimum essential medium (Sigma-Aldrich, G5154) supplemented with 15% fetal calf serum (Eurobio Abcys), 1% l-glutamine (Thermo Fisher Scientific, 25030024), 1% sodium pyruvate MEM (Thermo Fisher Scientific, 11360039), 1% MEM non-essential amino acids (Thermo Fisher Scientific, 11140035) 100 µM β-mercaptoethanol, 20 U ml⁻¹ leukaemia inhibitory factor (Miltenyi Biotec, premium grade) in 8% CO₂ at 37 °C. Cells were tested for mycoplasma contamination once a month and no contamination was detected. After piggyBac-enhancer transposition, cells were cultured in standard E14 medium supplemented with 2i (1 µM MEK inhibitor PDO35901 (Axon, 1408) and 3 µM GSK3 inhibitor CHIR 99021 (Axon, 1386)).

Zuin J., Roth G., Zhan Y., Cramard J., Redolfi J., Piskadlo E., Mach P., Kryzhanovska M., Tihanyi G., Kohler H., Eder M., Leemans C., van Steensel B., Meister P., Smallwood S, & Giorgetti L. (2022). Nonlinear control of transcription through enhancer–promoter interactions. Nature, 604(7906), 571-577.

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