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5 protocols using phenethyl acetate

1

Comprehensive Analytical Standards for Wine Aroma Profiling

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Chromatographically pure standards included ethyl acetate, ethyl isobutyrate, isobutyl acetate, butyl acetate, ethyl 2-methylbutyrate, 2-methyl-1-propanol, isoamyl acetate, 1-butanol, 3-methyl-1-butanol, ethyl hexanoate, hexyl acetate, propanoic acid, ethyl L(-)-lactate, 1-hexanol, cis-3-hexenol, ethyl octanoate, 1-heptanol, 2,3-butanediol, 1-octanol, isobutyric acid, diethyl succinate, L-α-terpineol, phenethyl acetate, hexanoic acid, benzyl alcohol, phenylethyl alcohol, octanoic acid, decanoic acid, ethyl lactate, ethyl decanoate (Sigma-Aldrich, Shanghai, China). Ultrapure 18.2 MΩ cm water used for these experiments was produced from the Milli-Q Ultrapure water system (Human Corp., Seoul, Korea). Absolute ethanol (analytically pure) was purchased from Sichuan Xilong Chemical Industry Co. Ltd. (Chengdu, Sichuan, CHN).
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2

Comprehensive Analysis of Volatile Compounds

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The standards of volatile compounds were purchased from Sigma-Aldrich (St. Louis, MO, USA), including isobutanol, 3-methyl-1-butanol, cis-3-hexen-1-ol, 1-hexanol, heptanol, octanol, phenethyl alcohol, 2,6-nonadien-1-ol, dodecanol, ethyl acetate, isoamyl acetate, ethyl lactate, ethyl hexanoate, ethyl octanoate, diethyl succinate, phenethyl acetate, ethyl decanoate, butanoic acid, hexanoic acid, octanoic acid, dodecanoic acid, octanal, (2e,6z)-2,6-nonadienal, benzaldehyde, furfural, citronellol, linalool, rose oxide, geraniol, nerol, β-damascenone, β-ionone, nerolidol, hexanal, guaiacol, 4-Ethylphenol, and 2-octanol which were used as the internal standard.
Folin–Ciocalteu reagent and sodium chloride were purchased from Beijing Chemical Works (Beijing, China). Potassium hydrogen tartrate were purchased at Kemiou Chemical Reagent Co. (Tianjin, China). Bovine serum albumin was obtained from Asahi Kasei Corporation (Tokyo, Japan). Deionized water (<18 MW resistance) was purified by using a Milli-Q purification system (Molecular, Chongqing, China). Bentonite, soybean protein and potassium metabisulfite were purchased from Lallemand Company (Lallemand, Toulouse, France).
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3

GC-MS Analysis of Volatile Compounds

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The analysis
was performed using Agilent 7890 A GC with an HP-5MS capillary column
(0.25 mm × 30 m × 0.25 μm) and a quadrupole mass spectrometer
Agilent 5975 C (Agilent, Santa Clara, CA, USA) as the detector. The
temperature conditions of the column were referenced by Yang et al.34 (link) The MS detection proceeded in electron impact
mode, and the ionization energy was 70 eV. The mass-scan range was
29–350 m/z at 2.88 scans/s.
Helium was the carrier gas at 1 mL/min.
Volatile compound identifications
were carried out by matching standard compounds and mass spectral
libraries (Wiley6 and NIST 98). A total of 17 standard compounds,
including 1-hexanol, 1-octen-3-ol, 2-ethylhexanol, benzaldehyde, benzoic
acid, benzyl acetate, benzyl alcohol, ethyl benzoate, geraniol, hexyl
acetate, linalool, nonanal, phenethyl acetate, phenylethyl alcohol,
prenyl acetate, terpineol, and β-pinene, and the internal standard
3-octanol was purchased from Sigma-Aldrich (Shanghai) Trading Co.,
Ltd. (Shanghai, China). The retention index values were derived from
alkane series (C7–C30) (Sigma, St. Louis,
MO, USA). All volatile compounds were quantified as 3-octanol equivalents,
and the results were expressed in microgram standard equivalents per
kilogram fresh weight (μg/kg FW).
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4

Preparation of Flour-based Noodles

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Flour was provided by the Hebei Jinshahe flour industry group. The flour had a moisture content of 13.04% ± 0.12 (w.b.) and protein content of 12.78%. The edible salt used in noodles comes from the local supermarket (Yangling, Shaanxi). The phenethyl acetate was purchased from Sigma-Aldrich (73747, St. Louis, MO, USA). All chemical reagents used in this study are analytical grade.
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5

Analytical Reagents for Scientific Research

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Riboflavin, NADPH, NADH, ethionamide, 4-phenyl-2-butanone, phenethyl acetate, 2octanone, hexyl acetate, 2-decanone, octyl acetate, 2-decanone, methyl undecanoate, decyl acetate, acetonitrile, ethyl acetate, methanol, n-hexane, and salts were purchased from Sigma-Aldrich (Italy).
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