Vegfr2 kdr kinase assay kit

Manufactured by BPS Biosciences

Sourced in United States

The VEGFR2 (KDR) kinase assay kit is a laboratory product designed to measure the enzymatic activity of the VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) protein. This kit provides the necessary components to perform an in vitro kinase assay, allowing researchers to quantify the activity of the VEGFR2 enzyme.

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Lab products found in correlation

Prism 7, by GraphPad (1 mentions) Akt kinase activity assay kit, by Abcam (1 mentions) Glomax 96 microplate luminometer, by Promega (1 mentions)

5 protocols using vegfr2 kdr kinase assay kit

VEGFR2 and Topoisomerase II Kinase Assay

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VEGFR2 (KDR) kinase assay kit “BPS Bioscience Corporation catalog#40325” and Topo isomearse II screening kit “TopoGEN, Inc., Columbus, OH, USA” were utilized to invetstiage the enzyme activity for the most active compounds following the manufacturer instructions. Inhibition of autophosphorylation by tested compounds was determined. The IC₅₀ values were determined using GraphPad Prism 7 software from curves depicting the percentage of inhibition using eight concentrations of each compound [43 (link),44 (link)].

Gomaa M.S., Ali I.A., El Enany G., El Ashry E.S., El Rayes S.M., Fathalla W., Ahmed A.H., Abubshait S.A., Abubshait H.A, & Nafie M.S. (2022). Facile Synthesis of Some Coumarin Derivatives and Their Cytotoxicity through VEGFR2 and Topoisomerase II Inhibition. Molecules, 27(23), 8279.

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Inhibition of VEGFR-2 Kinase Activity

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Compounds 3a, 3i, and 3l were tested for their ability to inhibit VEGFR-2 kinase activity using a VEGFR2 (KDR) kinase assay kit (BPS Bioscience, Corporation catalog # 40325) according to the manufacturer's instructions. The percentage inhibition of autophosphorylation by substances was estimated using the following equation^{62 (link)}:

Soliman D.H, & Nafie M.S. (2023). Design, synthesis, and docking studies of novel pyrazole-based scaffolds and their evaluation as VEGFR2 inhibitors in the treatment of prostate cancer. RSC Advances, 13(30), 20443-20456.

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VEGFR2 Kinase Activity Assay

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VEGFR2 TK activity was measured in vitro using the VEGFR2 (KDR) Kinase Assay Kit (BPS Bioscience, San Diego, CA, USA). Solo F–OH was tested at 20 μM according to the manufacturer’s instructions, determining the percentage of remaining TK activity after 45 min of incubation at 30 °C. Sunitinib 1 μM was used as positive control of inhibition in the same experimental conditions.

Carrillo P., Martínez-Poveda B., Cheng-Sánchez I., Guerra J., Tobia C., López-Romero J.M., Sarabia F., Medina M.Á, & Quesada A.R. (2019). Exploring the Antiangiogenic Potential of Solomonamide A Bioactive Precursors: In Vitro and In Vivo Evidences of the Inhibitory Activity of Solo F-OH During Angiogenesis. Marine Drugs, 17(4), 228.

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Enzyme Inhibition Assays for VEGFR-2 and AKT-1

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The nominated protein kinase activity inhibition by the novel compounds was determined quantitively by the VEGFR2 (KDR) Kinase Assay Kit (Catalog # 40325, BPS, Bioscience, San Diego, CA, USA) against VEGFR-2 [55 ,56 (link)] and Akt Kinase Activity Assay Kit (ab139436, ABCAM, USA) for AKT-1 enzyme on cell lysate according to the reported procedures [57 ,58 (link)]. The outcomes were expressed as IC₅₀ values (mean standard deviation) calculated from the dose–response curves and their linear regression equations. Sorafenib was used as the reference in the VEGFR-2 assay, and LY2780301 was used in the AKT assay.

Abdelnaby R.M., El-Malah A.A., FakhrEldeen R.R., Saeed M.M., Nadeem R.I., Younis N.S., Abdel-Rahman H.M, & El-Dydamony N.M. (2022). In Vitro Anticancer Activity Screening of Novel Fused Thiophene Derivatives as VEGFR-2/AKT Dual Inhibitors and Apoptosis Inducers. Pharmaceuticals, 15(6), 700.

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VEGFR2 Kinase Inhibition Assay

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VEGFR2 kinase activity was measured in vitro using the VEGFR2 (KDR) Kinase Assay kit (BPS Bioscience). Fasentin was tested at 100 µM according to the manufacturer’s instructions, determining the percentage of remaining kinase activity after 45 min incubation at 30 °C. 1 µM sunitinib was used as positive control of inhibition^{24 (link),25 (link)}. Data were collected using a GloMax-96 microplate luminometer (Promega).

Ocaña M.C., Martínez-Poveda B., Marí-Beffa M., Quesada A.R, & Medina M.Á. (2020). Fasentin diminishes endothelial cell proliferation, differentiation and invasion in a glucose metabolism-independent manner. Scientific Reports, 10, 6132.

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