Log In Sign up
The largest database of trusted experimental protocols

Human il 6

Manufactured by BD
Visit Supplier
Sourced in United States

Human IL-6 is a recombinant protein that functions as a cytokine. It is involved in the regulation of immune responses and inflammatory reactions.

Automatically generated - may contain errors

Lab products found in correlation

Mouse il 6, by BD (5 mentions) Porcine il 6, by R&D Systems (3 mentions) Mouse tnf α, by BD (3 mentions) Mouse il 6, by BioLegend (2 mentions) Human il 8, by BD (2 mentions) Mouse interferon β, by Cusabio (2 mentions) Human interferon β, by Cusabio (2 mentions) Human il 10, by BD (2 mentions) Human ifn β, by PBL Assay Science (2 mentions) Mouse ifn β, by PBL Assay Science (2 mentions) Human il 1β, by BD (2 mentions) Mouse mip 2, by R&D Systems (1 mentions) Csb e09889h, by R&D Systems (1 mentions) Csb e09890p, by Cusabio (1 mentions) L lactic acid, by Merck Group (1 mentions) Mcp 1 ccl 2 elisa kits, by BioLegend (1 mentions) Mouse il 13 elisa kit, by Thermo Fisher Scientific (1 mentions) Mcp 1 elisa kits, by BD (1 mentions) Recombinant human il 33, by BioLegend (1 mentions) Vegf elisa kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Human IL-6 ELISA kit (15 citations) Human IL-6 ELISA Set (14 citations) BD OptEIA™ Human IL-6 ELISA Set (11 citations) Rat anti-human IL-6 capture antibody (5 citations) BD OptEIA® Set Human IL-6 (4 citations) ELISA kits for human IL-6 (3 citations) Human IL-6 Flex Set (3 citations) Biotinylated rat anti-human IL-6 detection antibody (3 citations) Rat anti-human IL-6 antibodies (3 citations) BD OptEIA Human IL-6 ELISA kit II (3 citations)

12 protocols using human il 6

1

Cytokine Profiling in Immune Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA was used to measure cytokines as described previously [7 (link)]. For measuring cytokines in culture supernatants, the endometrial epithelial cells or bone marrow-derived cells were grown in 24 well plates at the cell density of 1.5 × 105/ml for MS74 [an immortalized human vaginal epithelial cell line kindly provided by Dr. Robert Schenken from Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio; ref: [16 (link)]] or 1 × 106/ml for bone marrow-derived neutrophils. Cells were stimulated with LPS (Cat# tlrl-3pelps), Pgp3, LL-37 or Pgp3/LL-37 complex at different concentrations as indicated in individual experiments for 24h. The supernatants with or without dilutions were used for cytokine measurement. The LL-37/Pgp3 complex was formed by pre-incubating the two together in cell culture medium for 30min at 37°C. ELISA kits were used for measuring mouse IL-6 (Cat# 431301, Biolegend, San Diego, CA), mouse MIP-2 (Cat# DY452, R&D systems, Minneapolis, MN), human IL-6 (Cat# 555220) and human IL-8 (Cat# 555244, both from BD Bioscience, San Jose, CA) respectively.
Hou S., Sun X., Dong X., Lin H., Tang L., Xue M, & Zhong G. (2018). Chlamydial plasmid-encoded virulence factor Pgp3 interacts with human cathelicidin peptide LL-37 to modulate immune response. Microbes and infection, 21(1), 50-55.
+ Open protocol
+ Expand
2

ELISA for Cytokine Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA was performed to detect the secreted IFNs and proinflammatory cytokines in cell culture supernatants. Mouse IL-6 (BD Biosciences, 555240), mouse interferon-β (CUSABIO, CSB-E04945m), human IL-6 (BD Biosciences, 555220), human interferon-β (CUSABIO, CSB-E09889h), porcine IL-6 (R&D Systems, P6000B), and porcine interferon-β (CUSABIO, CSB-E09890p) were used for the analysis according to the manufacturer’s protocols.
Ekanayaka P., Lee B.H., Weerawardhana A., Chathuranga K., Park J.H, & Lee J.S. (2021). Inhibition of MAVS Aggregation-Mediated Type-I Interferon Signaling by Foot-and-Mouth Disease Virus VP3. Viruses, 13(9), 1776.
+ Open protocol
+ Expand
3

Mouse Cytokine ELISA Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant mouse IL-3, SCF, and IL-33, recombinant human IL-33, as well as mouse IL-6, TNF, and MCP-1 (CCL-2) ELISA kits were purchased from BioLegend (San Diego, CA). Mouse MIP-1α (CCL-3) and VEGF ELISA kits were purchased from PeproTech (Rocky Hill, NJ). Mouse IL-13 ELISA kits were purchased from eBioscience (San Diego, CA). L-(+)-lactic acid and Sodium L-lactate were purchased from Sigma-Aldrich (St. Louis, MO). Human IL-6, TNF, and MCP-1 ELISA kits were purchased from BD OptEIA (BD Biosciences; Franklin Lakes, NJ).
Abebayehu D., Spence A.J., Qayum A.A., Taruselli M.T., McLeod J.J., Caslin H.L., Chumanevich A.P., Kolawole E.M., Paranjape A., Baker B., Ndaw V.S., Barnstein B.O., Oskeritzian C.A., Sell S.A, & Ryan J.J. (2016). Lactic Acid Suppresses IL-33-mediated Mast Cell Inflammatory Responses via Hypoxia Inducible Factor (HIF)-1α-dependent miR-155 Suppression. Journal of immunology (Baltimore, Md. : 1950), 197(7), 2909-2917.
+ Open protocol
+ Expand
4

Multiplex Cytokine Profiling in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Human IL-1ra, Human IL-6, Human IL-10, Human IL-13, Human Eotaxin, Human IFN-γ, Human SCF and Human TRAIL (BD Biosciences, San Jose, CA, United States) ELISA kits were used to measure plasma concentration of IL-1ra, IL-6, IL-10, IL-13, Eotaxin, IFN-γ, SCF and TRAIL, respectively. We used assay protocol and sample dilution in accordance with the manufacturers’ instructions.
Ma Q., Chen R., Zeng J., Lei B., Ye F., Wu Q., Li Z., Zhan Y., Liu B., Chen B, & Yang Z. (2022). Investigating the effects of Liushen Capsules (LS) on the metabolome of seasonal influenza: A randomized clinical trial. Frontiers in Pharmacology, 13, 968182.
+ Open protocol
+ Expand
5

Cytokine Secretion Profiling by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse sera, tissue homogenates, or cell supernatants were used to examine level of cytokine secretion by ELISA. Commercial kits as followings were used according to manufacturer's protocols: mouse IFN‐β (PBL Interferon Source), mouse IL‐6 (BD Biosciences), mouse IL‐12 (BD Biosciences), mouse TNF‐α (BD Biosciences), mouse CCL5 (R&D Systems), mouse CXCL10 (R&D Systems), mouse IL‐1β (BD Biosciences), human IFN‐β (PBL interferon source), and human IL‐6 (BD Biosciences).
Chathuranga K., Kim T., Lee H., Park J., Kim J., Chathuranga W.A., Ekanayaka P., Choi Y.J., Lee C., Kim C., Jung J.U, & Lee J. (2020). Negative regulation of NEMO signaling by the ubiquitin E3 ligase MARCH2. The EMBO Journal, 39(21), e105139.
+ Open protocol
+ Expand
6

Cytokine Production Analysis by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA was used to detect the production of pro-inflammatory cytokines and type I interferon from cells. After infection, treatment and transfection of stimulants, cell supernatant was collected and analyzed cytokine production levels. Mouse IFN-α (PBL interferon source), mouse IFN-β (PBL interferon source), mouse IL-6 (BD biosciences) and mouse TNF-α (BD biosciences), human IFN-β (PBL interferon source) and human IL-6 (BD biosciences) were used for analysis according to manufacturer’s protocol.
Kim J.H., Park M.E., Nikapitiya C., Kim T.H., Uddin M.B., Lee H.C., Kim E., Ma J.Y., Jung J.U., Kim C.J, & Lee J.S. (2017). FAS-associated factor-1 positively regulates type I interferon response to RNA virus infection by targeting NLRX1. PLoS Pathogens, 13(5), e1006398.
+ Open protocol
+ Expand
7

Cytokine Detection in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enzyme-linked immunosorbent assay (ELISA) was performed to detect secreted inflammatory cytokines in cell culture supernatants. Human IL-6 (BD Biosciences, 555220), human interferon-β (CUSABIO, CSB-E09889h), mouse IL-6 (BD Biosciences, 555240), mouse interferon-β (CUSABIO, CSB-E04945m), mouse interferon-α (PBL Assay Science, 42120-1), mouse TNF-α (BD Biosciences, 555268), porcine IL-6 (R&D Systems, P6000B), and porcine IFN-α (CUSABIO, CSB-E07328p) were used for analysis according to the manufacturer’s protocols.
Ekanayaka P., Shin S.H., Weeratunga P., Lee H., Kim T.H., Chathuranga K., Subasinghe A., Park J.H, & Lee J.S. (2021). Foot-and-Mouth Disease Virus 3C Protease Antagonizes Interferon Signaling and C142T Substitution Attenuates the FMD Virus. Frontiers in Microbiology, 12, 737031.
+ Open protocol
+ Expand
8

Cytokine Profiling in Viral Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To measure type-I interferon and proinflammatory cytokines, mouse IFN-β (PBL interferon source), mouse IL-6 (BD Biosciences), human IFN-β (PBL interferon source) and human IL-6 (BD Biosciences) ELISA kits were used. After infection of virus to cells, the levels of secreted cytokines were measured in supernatant of infected cell. For mice, plasma levels of type-I interferon and proinflammatory cytokines were analysed.
Yoo Y.S., Park Y.Y., Kim J.H., Cho H., Kim S.H., Lee H.S., Kim T.H., Sun Kim Y., Lee Y., Kim C.J., Jung J.U., Lee J.S, & Cho H. (2015). The mitochondrial ubiquitin ligase MARCH5 resolves MAVS aggregates during antiviral signalling. Nature Communications, 6, 7910.
+ Open protocol
+ Expand
9

Quantifying ACE2 and Cytokine Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
ACE2 and ILs levels were measured by commercially available sandwich enzyme-linked immunosorbent assays (ELISAs) following manufacturer’s instructions. The following ELISA kits were employed: human IL-6 (BD Biosciences cat. 555220); human IL-8 (BD Biosciences cat. 555224); human IL-1β (BD Biosciences cat. 557953); human IL-10 (BD Biosciences cat. 555157), and human ACE2 duoset kit (R&D SYSTEMS cat. DY933–05).
Silva M.G., Corradi G.R., Pérez Duhalde J.I., Nuñez M., Cela E.M., Gonzales Maglio D.H., Brizzio A., Salazar M.R., Espeche W.G, & Gironacci M.M. (2022). Plasmatic renin-angiotensin system in normotensive and hypertensive patients hospitalized with COVID-19. Biomedicine & Pharmacotherapy, 152, 113201.
+ Open protocol
+ Expand
10

In Vitro Activation and Sensitization of T and B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Before sensitization in vitro, T or B cells were activated respectively with immobilized anti-human CD3 (BD Pharmingen, San Jose, CA)(2 μg/ml) and anti-human CD28 (BD Pharmingen) (2 μg/ml) in complete medium (CM) containing 150 IU/ml hrIL-2 (Norvtis, Princeton, NJ) or 5 μg/ml lipopolysaccharide (LPS, Sigma-Aldrich, Atlanta, GA) plus 2 μg/ml anti-human CD40 (BD Pharmingen) at 37°C with 5% CO2 for 24 h. DCs were generated from the CD3−CD19− PBMCs of the same patient by differentiation with 25 μg/ml human IL-4 (BD Pharmingen) and 50 μg/ml human GM-CSF (BD Pharmingen) in CM for 10 days. Half of the medium was changed every other day from the third culture day. DCs were then pulsed with ALDHhigh or ALDHlow tumor cell lysate at the ratio of DC : tumor cell= 3:1 in CM containing 100 IU/ml human IL-6 (BD Pharmingen), 10 ng/ml human IL-1β (BD Pharmingen) and 10 ng/ml human TNF-α (BD Pharmingen) for 4-6 h. The lysate pulsed-DCs were then added to T or B cells under activation and incubated for another 24 h for sensitization.
Prince M.E., Zhou L., Moyer J.S., Tao H., Lu L., Owen J., Etigen M., Zheng F., Chang A.E., Xia J., Wolf G., Wicha M.S., Huang S., Ren X, & Li Q. (2016). Evaluation of the immunogenicity of ALDHhigh human head and neck squamous cell carcinoma cancer stem cells in vitro. Oral oncology, 59, 30-42.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!