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Ivis 200 system

Manufactured by Novus Biologicals
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The IVIS 200 system is an in vivo imaging system designed for non-invasive, real-time visualization and quantification of biological processes in live animals. It utilizes bioluminescent and fluorescent reporters to enable the study of gene expression, cell trafficking, and a variety of other applications.

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Lab products found in correlation

Pvdf membrane, by PerkinElmer (2 mentions) Pathscan intracellular signaling array kit, by Cell Signaling Technology (2 mentions) Lysis buffer, by Cell Signaling Technology (2 mentions) Perfection v600 photo scanner, by Epson (2 mentions)

2 protocols using ivis 200 system

1

Quantitative Immunoblotting and Signaling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunoreactivity studies, tissue samples were diluted to 100 mg/mL in lysis buffer (#7018s, Cell Signaling Technology) and subsequently homogenized, sonicated, and spun at 3.5K RPM for 1 min. Lysate samples were then screened against an array of target-specific capture antibodies using PathScan Intracellular Signaling Array Kits (#7323, Cell Signaling Technology) in accordance with the manufacturer’s instructions and developed using HyBlot CL autoradiography film (#e3012, Denville Scientific Inc.). Processed films were digitized using an Epson Perfection V600 Photo scanner and the relative pixel intensities for each blot quantified using the image processing software ImageJ. Complementary western blot studies were performed by loading 20-40 µg of protein into 7.5-12% SDS PAGE gels, transferred to PVDF membranes (PerkinElmer), and resolved by electrophoresis. Membranes were blocked overnight at 4°C in trisbuffered saline 0.1% Tween-20 (TBST) containing 5% w/v nonfat dry milk powder and subsequently incubated with antibodies to p-ERK 1/2 Thr202/Tyr204 (Cell Signaling, 4370), pS6 (Cell Signaling, #4858) LC3B, (anti LC3B, Cell signaling 2775), or b-tubulin (Novus Biologicals, NB600-936); Membrane chemiluminescence was imaged on a Xenogen IVIS 200 system.
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
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2

Quantitative Immunoblotting and Signaling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunoreactivity studies, tissue samples were diluted to 100 mg/mL in lysis buffer (#7018s, Cell Signaling Technology) and subsequently homogenized, sonicated, and spun at 3.5K RPM for 1 min. Lysate samples were then screened against an array of target-specific capture antibodies using PathScan Intracellular Signaling Array Kits (#7323, Cell Signaling Technology) in accordance with the manufacturer’s instructions and developed using HyBlot CL autoradiography film (#e3012, Denville Scientific Inc.). Processed films were digitized using an Epson Perfection V600 Photo scanner and the relative pixel intensities for each blot quantified using the image processing software ImageJ. Complementary western blot studies were performed by loading 20-40 µg of protein into 7.5-12% SDS PAGE gels, transferred to PVDF membranes (PerkinElmer), and resolved by electrophoresis. Membranes were blocked overnight at 4°C in trisbuffered saline 0.1% Tween-20 (TBST) containing 5% w/v nonfat dry milk powder and subsequently incubated with antibodies to p-ERK 1/2 Thr202/Tyr204 (Cell Signaling, 4370), pS6 (Cell Signaling, #4858) LC3B, (anti LC3B, Cell signaling 2775), or b-tubulin (Novus Biologicals, NB600-936); Membrane chemiluminescence was imaged on a Xenogen IVIS 200 system.
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
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