Fv1000mpe multi photon laser scanning microscope

Manufactured by Zeiss

The FV1000MPE is a multi-photon laser scanning microscope manufactured by Zeiss. It is designed to perform high-resolution imaging of living samples by utilizing the principles of multi-photon excitation. The core function of the FV1000MPE is to enable the capture of detailed, three-dimensional images of biological samples.

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Lab products found in correlation

Plan apochromat 20x, by Zeiss (2 mentions) Xlpln 25x wmp2, by Zeiss (2 mentions) Zen blue, by Zeiss (2 mentions) Premiere pro, by Adobe (1 mentions)

2 protocols using fv1000mpe multi photon laser scanning microscope

Imaging of GFP/RFP-labeled Cells

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We used an upright microscope set-up (Olympus FV1000MPE multi-photon laser scanning microscope or Zeiss 880, both with Spectra-Physics Mai Tai DeepSee 2-photon lasers) with water-immersion lens (Olympus XLPLN 25X WMP2 or Zeiss Plan-Apochromat 20X). The objective was immersed directly in the culture medium for imaging. The fluorophores used were all GFP or RFP derivatives, therefore the excitation wavelength was tuned between 925 and 935 nm. Laser power never exceeded 15%. Zen Blue (Zeiss) and ImageJ software was used to analyze movies. The Bleach Correction and Manual Tracking plugins were used to correct photobleaching and to track cells. The Correct 3D Drift plugin was used to correct for movements caused by tissue contraction. Adobe Photoshop (v21.1.3) and Adobe Premiere Pro (v14.2) were used to annotate and edit movies. Figures were generated using Adobe Illustrator (v24.1.3).

Bostock M.P., Prasad A.R., Chaouni R., Yuen A.C., Sousa-Nunes R., Amoyel M, & Fernandes V.M. (2020). An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. Frontiers in Cell and Developmental Biology, 8, 590094.

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Intravital 2-Photon Microscopy Imaging

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We used an upright microscope set-up (Olympus FV1000MPE multi-photon laser scanning microscope or Zeiss 880, both with Spectra-Physics MaiTai DeepSee 2-photon lasers) with waterimmersion lens (Olympus XLPLN 25X WMP2 or Zeiss Plan-Apochromat 20X). The fluorophores used were all GFP or RFP derivatives, therefore the excitation wavelength was tuned between 925nm and 935 nm. Laser power never exceeded 15 %. Zen Blue (Zeiss) and ImageJ software was used to analyze movies. The Bleach Correction and Manual Tracking plugins were used to correct photobleaching and to track cells. The Correct 3D Drift plugin was used to correct for movements caused by tissue contraction. Adobe Photoshop (v21.1.3) and Adobe Premiere Pro (v14.2) were used to annotate and edit movies. Figures were generated using Adobe Illustrator (v24.1.3).

Bostock M.P., Prasad A.R., Chaouni R., Yuen A.C., Sousa‐Nunes R., Amoyel M., & Fernandes V.M. (2020). An immobilization technique for long-term time-lapse imaging of explantedDrosophilatissues.

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