Freestyle freedom light

Manufactured by Abbott

Sourced in United States

The FreeStyle Freedom Lite is a compact and portable blood glucose monitoring system. It is designed to measure blood glucose levels from a small blood sample. The device provides accurate and reliable results to help individuals manage their diabetes.

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Lab products found in correlation

Chod pap, by Roche (2 mentions) Gpo pap, by Roche (2 mentions) Mcp 1 ccl2, by R&D Systems (1 mentions) Kma0021, by Thermo Fisher Scientific (1 mentions) Hk205 02, by Hycult Biotech (1 mentions) Human insulin, by Eli Lilly (1 mentions) Reflotron plus, by Roche (1 mentions)

Close spelling variants of this product

FreeStyle Freedom

4 protocols using freestyle freedom light

Plasma Biomarkers in Fasting Mice

Cited 2 times

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Fasting (5 h) blood samples were taken from the tail vein to prepare EDTA plasma. Blood glucose was determined during blood sampling using a hand-held glucometer (Freestyle Freedom Light, Abbott Laboratories, Lake Bluff, IL, United States). Plasma insulin, leptin, adiponectin, cholesterol, triacylglycerols, ALT, CK-18M30 were determined as described (16 (link)). ELISA assays were used to measure plasma serum amyloid A (SAA; KMA0021 Thermo Fisher Scientific, Waltman, United States), MCP-1/CCL2 (DY479 R&D, Minneapolis, United States), MIF (DY1978 R&D, Fetuin A (DY1563 R&D) and LPS binding protein (LBP; HK205-02 Hycult Biotech, Uden, Netherlands) according to manufacturer’s protocol.
Lipoprotein profiles were analyzed by first separating lipoproteins into fractions using fast protein liquid chromatography (FPLC) with an AKTA apparatus (Pharmacia, Roosendaal, Netherlands), as previously described (17 (link), 18 (link)). Subsequently, total cholesterol and triacylglycerols were measured in the fractions collected for profiling with enzymatic assays (Roche diagnostics, Basel, CHF) (19 (link)–21 (link)).

Gart E., Salic K., Morrison M.C., Giera M., Attema J., de Ruiter C., Caspers M., Schuren F., Bobeldijk-Pastorova I., Heer M., Qin Y, & Kleemann R. (2022). The Human Milk Oligosaccharide 2′-Fucosyllactose Alleviates Liver Steatosis, ER Stress and Insulin Resistance by Reducing Hepatic Diacylglycerols and Improved Gut Permeability in Obese Ldlr-/-.Leiden Mice. Frontiers in Nutrition, 9, 904740.

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Insulin Tolerance Test in Mice

Cited 1 time

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An insulin tolerance test was performed as previously described [16] (link). Briefly, after 6 h of starvation, mice were intraperitoneally injected with human insulin (2.0 U/kg; Eli Lilly, Indianapolis, IN, USA). Blood was collected from the tail vein after the insulin application and glucose levels were measured using a Free Style Freedom Light (Abbott Japan, Tokyo, Japan).

Saito N., Kimura S., Miyamoto T., Fukushima S., Amagasa M., Shimamoto Y., Nishioka C., Okamoto S., Toda C., Washio K., Asano A., Miyoshi I., Takahashi E, & Kitamura H. (2017). Macrophage ubiquitin-specific protease 2 modifies insulin sensitivity in obese mice. Biochemistry and Biophysics Reports, 9, 322-329.

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Comprehensive Biomarker Measurements Protocol

Cited 2 times

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Blood glucose was measured during blood sampling with a hand-held glucometer (Freestyle Freedom Light, Abbott Laboratories, Lake Bluff, IL, USA). Plasma insulin was measured by ELISA (Chrystal Chem Inc., Downers Grove, IL, USA). Plasma cholesterol and plasma triglyceride levels were measured in freshly prepared plasma using enzymatic assays (CHOD-PAP and GPO-PAP, respectively; Roche Diagnostics, Almere, The Netherlands). Plasma TIMP-1 was measured in terminal plasma by ELISA (R&D systems, Abingdon, UK). All these assays were performed according to the manufacturers’ instructions. Extracellular matrix turnover markers PRO-C3 and PRO-C4 for type 3 and type 4 collagen formation and C4M and C6M for metalloproteinase-mediated degradation of type 4 and type 6 collagen, respectively [47 (link),48 (link),49 (link),50 (link)], were measured in terminal serum using competitive ELISAs (Nordic Bioscience, Herlev, Denmark).

Morrison M.C., Gart E., van Duyvenvoorde W., Snabel J., Nielsen M.J., Leeming D.J., Menke A, & Kleemann R. (2022). Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr−/−.Leiden Mice. International Journal of Molecular Sciences, 23(4), 2325.

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Comprehensive Biochemical Profiling Protocol

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Blood glucose was determined during blood sampling using a hand-held glucometer (Freestyle Freedom Light, Abbott Laboratories, Lake Bluff, IL, USA). Plasma insulin was determined by ELISA (Chrystal Chem Inc., Downers Grove, IL, USA). Plasma lipids (total cholesterol and triglycerides) were assayed in freshly prepared EDTA-plasma using commercially available enzymatic assays (CHOD-PAP and GPO-PAP respectively; Roche Diagnostics, Almere, the Netherlands). Plasma serum amyloid A (SAA) was measured by ELISA (Invitrogen/Thermo Fisher Waltham, MA, USA). Plasma alanine aminotransferase (ALT) was assessed using a spectrophotometric activity assay (Reflotron-plus, Roche Diagnostics). All these analyses were performed according to the manufacturers’ instructions.

Mueller A.M., Kleemann R., Gart E., van Duyvenvoorde W., Verschuren L., Caspers M., Menke A., Krömmelbein N., Salic K., Burmeister Y., Seilheimer B, & Morrison M.C. (2021). Cholesterol Accumulation as a Driver of Hepatic Inflammation Under Translational Dietary Conditions Can Be Attenuated by a Multicomponent Medicine. Frontiers in Endocrinology, 12, 601160.

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