Enhanced chemiluminescence ecl western blotting detection system

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was purchased from Sigma–Aldrich (Saint Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), minimum essential medium alpha (α-MEM), and fetal bovine serum (FBS) were purchased from Welgene Bioscience (Daegu, Korea). Primary antibodies for iNOS, COX-2, HO-1, and EGFR rabbit polyclonal antibodies were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). The enhanced chemiluminescence (ECL) Western blotting detection system was obtained from Advansta Inc. (San Jose, CA, USA). The HPLC-grade acetonitrile and water were obtained from Honeywell-Burdick and Jackson (Muskegon, MI, USA). The TNF-α and IL-6 ELISA (enzyme-linked immunosorbent assay) kits were purchased from R&D Systems (Minneapolis, MN, USA). Lipopolysaccharide isolated from P. gingivalis (PG-LPS) was purchased from InvivoGen (San Diego, CA, USA). Alizarin Red S and tin protoporphyrin IX (SnPP) were obtained Sigma–Aldrich (St. Louis, MO, USA). n-Hexane, ethyl acetate, n-butanol, chloroform, and MeOH were purchased from Daejung Chemicals and Metals CO. LTD. (Siheung, Korea).

Recombinant soluble RANKL ligand (sRANKL) was acquired from PeproTech EC Ltd. (London, UK). An Acid Phosphatase Assay Kit, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), leukocyte acid phosphatase, 4-6-diamidino-2-phenylindole (DAPI), ascorbic acid, and β-glycerophosphate were purchased from Sigma-Aldrich Fine Chemicals (Saint Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), minimum essential medium alpha (α-MEM), and fetal bovine serum (FBS) were purchased from Welgene Bioscience (Daegu, Korea). TRIZOL reagent was purchased from JBI (Seoul, Korea) and TOPscript™ RT DryMIX (dT18 plus) was purchased from Enzynomics (Daejeon, Korea). TB Green^® Pre-mix Ex Taq™ II (Tli RNaseH Plus) was purchased from Takara (Tokyo, Japan). Primary antibodies for phospho-JNK, JNK, phospho-p38, p38, phospho-ERK, ERK, BIP, p-eIF2α, eIF2α, and rabbit polyclonal antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Catalase (CAT) and superoxide dismutase (SOD) antibodies were acquired from BD biosciences (San Jose, CA, USA). An anti-nuclear factor of activated T cells-c1 (NFATc1) antibody was acquired from BD biosciences (San Jose, CA, USA). The enhanced chemiluminescence (ECL) Western blotting detection system was purchased from Advansta Inc. (San Jose, CA, USA).