3 morpholinopropanesulfonic acid mops

Manufactured by Merck Group

3-morpholinopropanesulfonic acid (MOPS) is a buffering agent commonly used in biological and biochemical applications. It maintains a stable pH range between 6.5 and 7.9, making it suitable for a variety of experiments and procedures.

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Close spelling variants of this product

MOPS acid (6 citations) Morpholinopropanesulfonic acid (MOPS) (3 citations) 3-morpholinopropanesulfonic acid (2 citations)

2 protocols using 3 morpholinopropanesulfonic acid mops

Protein Purification and Analysis Protocol

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Dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium bromide (HNSB), deuterium oxide, pepsin, imidazole, 3-morpholinopropanesulfonic acid (MOPS), potassium acetate, potassium bromide, urea, zinc sulfate, deuterium oxide, tris(2-carboxyethyl)phosphine (TCEP), and dithiothreitol (DTT) were obtained from Sigma-Aldrich (St. Louis, MO). urea was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Trypsin was purchased from Promega (Madison, WI). Tris(hydroxymethyl)-aminomethane (Tris) and tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) were purchased from EM Science (Gladstone, NJ). Human β2m that was purified from human urine was purchased from Lee Biosolutions (St. Louis, MO). Ammonium acetate, methanol, acetonitrile, glacial acetic acid, copper sulfate, and nickel sulfate were obtained from Fisher Scientific (Fair Lawn, NJ). Centricon molecular weight cutoff (MWCO) filters were obtained from Millipore (Burlington, MA). Deionized water was prepared from a Millipore (Burlington, MA) Simplicity 185 water purification system.

Borotto N.B., Zhang Z., Dong J., Burant B, & Vachet R.W. (2017). Increased β-Sheet Dynamics and D-E Loop Repositioning are Necessary for Cu(II)-Induced Amyloid Formation by β-2-Microglobulin. Biochemistry, 56(8), 1095-1104.

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Fluorescent DNA-Enzyme Interaction Assay

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DNA conjugated with carboxyfluorescein (FAM) at the 3′ terminus (DNA-G), carboxytetramethylrhodamine (TAMRA) at the 3′ terminus (DNA-Y), and indodicarbocyanine (Cy5) at the 5′ terminus (DNA-R) were synthesized and purified by Eurofins Genomics (Ebersberg, Germany). Pepsin from porcine stomach mucosa (Pep), thrombin from bovine plasma (ThrB), elastase from porcine pancreas (Ela), α-chymo trypsin from bovine pancreas (Chy), proteinase K from tritirachium album (Pro), papain from papaya latex (Pap), trypsin from bovine pancreas (TryB), trypsin from porcine pancreas (TryP), α₁-antitrypsin from human plasma (AAT), 2-morpholinoethanesulfonic acid (MES), and 3-morpholinopropanesulfonic acid (MOPS) were purchased from Sigma-Aldrich, Co., LLC. (St. Louis, MO, USA). Thrombin from human plasma (ThrH) was purchased from Haematologic Technologies Inc. (Essex, VT, USA). Human serum was purchased from Cosmo Bio (Tokyo, Japan).

Okada M., Sugai H., Tomita S, & Kurita R. (2020). A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set. Sensors (Basel, Switzerland), 20(18), 5110.

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