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Mb162

Manufactured by Olympus

The MB162 is a high-precision laboratory microscope designed for detailed scientific observation. It features a stable optical system, coarse and fine focusing controls, and a range of objective lenses for versatile magnification capabilities. The core function of the MB162 is to enable clear and detailed visual inspection of samples under high magnification.

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3 protocols using mb162

1

Endoscopic Imaging System for Mucosal Evaluation

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The endoscopy system used in the prospective trial consisted of a video processor (CV-260SL; Olympus Medical Systems, Tokyo, Japan) and a light source (CLV-260SL; Olympus Medical Systems) that worked in both the high resolution WLE and NBI modes. NBI uses narrow-band short-wavelength light (400 – 430 and 525 – 555 nm) to contrast vascular architecture and surface structure of the superficial mucosa 4 (link). Zoom videogastroscopy that can achieve a maximum of 80-fold optical magnification was used (GIF-Q240Z, GIF-H260Z and GIF-FQ260Z; Olympus Medical Systems). A soft black hood (MB162 or MB46; Olympus Medical Systems) was attached to the tip of the endoscope. The structure enhancement of the endoscopic video processor was set to B-mode level 4 or 6 for WLE and to B-mode level 8 for magnifying NBI. The color mode was set to level 1.
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2

Magnifying Endoscopy with Narrow-Band Imaging

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The endoscopy system consisted of a video processor (CV-260SL; Olympus Co., Tokyo, Japan) and a light source (CLV-260SL; Olympus Co.) that worked in both the high-resolution white-light imaging and NBI modes. NBI illuminates narrow-banded short-wavelength light (400 – 430 and 525 – 555 nm) to contrast the vascular architecture and surface structure of the superficial mucosa
6 (link)
. Magnifying endoscopy that can achieve a maximum 80-fold optical magnification was used (GIF-Q240Z, GIF-H260Z, and GIF-FQ260Z; Olympus Co.). A soft black hood (MB162 or MB46; Olympus Co.) was attached to the tip of the endoscope to enable the endoscopist to maintain an adequate distance for maximal magnification of the endoscopic image. The structural enhancement of the endoscopic video processor was set to B-mode level 4 or 6 for white-light endoscopy and to B-mode level 8 for M-NBI. The color mode was set at level 1.
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3

Magnifying Endoscopy with NBI Imaging

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The endoscopic procedure was performed using a CV-260 video system (OLYMPUS Co., Tokyo, Japan) and a magnifying endoscope (Model H260Z; OLYMPUS). To obtain a clear view using ME-NBI, a black rubber attachment (MB-162; OLYMPUS) was fitted to the tip of the videoendoscope to ensure an appropriate distance between the lens and the mucosal surface. A single experienced endoscopist (M.K.) performed the endoscopic procedure. Before treatment of the carcinoma, the videoendoscope was inserted into the stomach, and the gastric disease was visualized using standard and magnifying views. ME-NBI observations were undertaken approaching the maximum range, and the images (approximately 10 – 50 images) were saved in a digital filing system. If there were several types of microvessel in the carcinoma, we analyzed the area with major microvascular forms in the lesion. The uninvolved mucosa surrounding the carcinoma was also examined using ME-NBI.
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