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Ndb0385

Manufactured by Bio Basic
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The NDB0385 is a laboratory equipment product manufactured by Bio Basic. It is a device designed for the purpose of measuring and analyzing specific parameters or properties of samples within a controlled laboratory setting. The core function of this product is to provide accurate and reliable data to support scientific research and analysis.

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Lab products found in correlation

M6250, by Merck Group (2 mentions) Hep001, by Bio Basic (2 mentions) Sb0476, by Bio Basic (2 mentions) Sov664, by Bio Basic (2 mentions) Ad0153, by Bio Basic (2 mentions) Ldj691, by Bio Basic (2 mentions) Leupeptin, by Bio Basic (2 mentions)

2 protocols using ndb0385

1

Protein Extraction from Flash-Frozen Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250). Immunoblots are representative of 3 independent experiments.
Turpin J., Ling C., Crosby E.J., Hartmann Z.C., Simond A.M., Chodosh L.A., Rennhack J.P., Andrechek E.R., Ozcelik J., Hallett M., Mills G.B., Cardiff R.D., Gray J.W., Griffith O.L, & Muller W.J. (2016). The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment. Oncogene, 35(47), 6053-6064.
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2

Protein Extraction from Flash-Frozen Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250). Immunoblots are representative of 3 independent experiments.
Turpin J., Ling C., Crosby E.J., Hartmann Z.C., Simond A.M., Chodosh L.A., Rennhack J.P., Andrechek E.R., Ozcelik J., Hallett M., Mills G.B., Cardiff R.D., Gray J.W., Griffith O.L, & Muller W.J. (2016). The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment. Oncogene, 35(47), 6053-6064.
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