Log In Sign up
The largest database of trusted experimental protocols

Ab01584

Manufactured by Abcam
Visit Supplier

Ab01584 is a high-quality laboratory equipment product designed for use in scientific research. The core function of this product is to facilitate specific experimental procedures, but a detailed description cannot be provided while maintaining an unbiased and entirely factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab256454, by Abcam (2 mentions) Sc 13596, by Santa Cruz Biotechnology (2 mentions) Pa5 17097, by Thermo Fisher Scientific (2 mentions) Sc 84694, by Santa Cruz Biotechnology (2 mentions) T4026, by Merck Group (2 mentions) Protease inhibitor, by Merck Group (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Enhanced chemiluminescence system, by Cytiva (1 mentions)

2 protocols using ab01584

1

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA-buffer [1% Nonident P-40, 50 mmol/L Tris-HCl (pH 7.4), and 150 mmol/L NaCl] supplemented with protease inhibitors (Sigma). Protein lysates were subjected to 6% SDS-PAGE, transferred to nitrocellulose membrane (Bio-Rad), and incubated with anti-Enpp1 (PA5-17097, Invitrogen), anti-Epas1 (sc-13596, Santa Cruz, RRID:AB_627525), anti-Srpx2 (Ab01584, Abcam), anti-Apcdd1 (sc-84694, Santa Cruz, RRID:AB_2057638), anti-Hp (ab256454, Abcam), and anti-tubulin antibody (T4026, Sigma-Aldrich). Bands were developed with enhanced chemiluminescence system (Amersham Bioscience).
Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
+ Open protocol
+ Expand
2

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA-buffer (1% Nonident P-40, 50 mM Tris-HCl (pH 7.4), and 150 mM NaCl) supplemented with protease inhibitors (Sigma, St. Louis, MO). Protein lysates were subjected to 6% SDS-PAGE, transferred to nitrocellulose membrane (Bio-Rad) and incubated with anti-Enpp1 (PA5-17097, Invitrogen), anti-Epas1 (sc-13596, Santa Cruz, RRID:AB_627525), anti-Srpx2 (Ab01584, abcam), anti-Apcdd1 (sc-84694, Santa Cruz, RRID:AB_2057638) and anti-Hp (ab256454, abcam) and anti-Tubulin antibody (T4026, Sigma-Aldrich). Bands were developed with enhanced chemiluminiscence (ECL) system (Amersham Bioscience, UK).
Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!