Trastuzumab (Herceptin®, Roche, binds HER2); pertuzumab (Perjeta®, Roche, binds HER2); TS2/16.2.1 mouse monoclonal produced in-house from hybridoma (ATCC Cat# HB-243, binds integrin β1); BIIG2 rat monoclonal produced in-house from hybridoma (DSHB Antibody Registry ID: AB_528155, binds α5 integrin); ab528 mouse monoclonal produced in-house from hybridoma (ATCC Cat# HB-8509 binds: epidermal growth factor receptor EGFR);Alexa Fluor 546 conjugated goat anti-Mouse IgG (H + L), highly cross-adsorbed (ThermoFisher cat# A-11030); Alexa Fluor 647 conjugated Goat anti-Mouse IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21235); Alexa Fluor 546 conjugated Goat anti-Human IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21089); Alexa Fluor 647 conjugated Goat anti-Human IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21445);Alexa Fluor 647 conjugated Goat anti-Rat IgG (H + L) highly cross-adsorbed (ThermoFisher cat# A-21247).
Alexa fluor 546 conjugated goat anti mouse igg h l
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 546 conjugated goat anti-Mouse IgG (H + L) is a secondary antibody used for fluorescent detection in immunoassays. It is produced by conjugating Alexa Fluor 546 dye to goat anti-mouse IgG antibodies that recognize the heavy and light chains of mouse immunoglobulins.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 conjugated goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 546 conjugated goat anti human igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated goat anti rat igg h l, by Thermo Fisher Scientific (1 mentions)
Herceptin, by Roche (1 mentions)
Perjeta, by Roche (1 mentions)
2 protocols using alexa fluor 546 conjugated goat anti mouse igg h l
1
Multiparametric Imaging of Cell Surface Receptors
2
Viral Antigen Expression in Tissue Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To identify cell types that expressed the viral antigens, double staining of a single
tissue section was conducted using the immunofluorescent antibody technique [21 (link)]. To evaluate the relationship between viral
antigen-positive cells and MCs, combined staining with anti-P and CK 20 antibodies was
done. To block nonspecific reactions, sections were treated with 10% normal goat serum.
The primary antibodies were diluted as described above. FITC-conjugated goat anti-rabbit
IgG (H+L) (Southern Biotechnology Associates, Inc., Birmingham, AL, USA) and Alexa Fluor
546-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Inc., Waltham, MA,
USA) were used as secondary antibodies at 1:200 dilutions. DAPI (Thermo Fisher Scientific)
was used for counterstaining.
tissue section was conducted using the immunofluorescent antibody technique [21 (link)]. To evaluate the relationship between viral
antigen-positive cells and MCs, combined staining with anti-P and CK 20 antibodies was
done. To block nonspecific reactions, sections were treated with 10% normal goat serum.
The primary antibodies were diluted as described above. FITC-conjugated goat anti-rabbit
IgG (H+L) (Southern Biotechnology Associates, Inc., Birmingham, AL, USA) and Alexa Fluor
546-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Inc., Waltham, MA,
USA) were used as secondary antibodies at 1:200 dilutions. DAPI (Thermo Fisher Scientific)
was used for counterstaining.
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