Clone m1

Manufactured by Cell Marque

The Clone M1 is a laboratory equipment product. It is designed for specific functions within a laboratory setting. The core function of the Clone M1 is to perform designated tasks required for laboratory procedures.

Automatically generated - may contain errors

Lab products found in correlation

Clone epr3947, by Roche (1 mentions) Iview dab detection kit, by Roche (1 mentions)

2 protocols using clone m1

Automated Immunohistochemistry for MMR Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC was performed using an immunostainer (automated slide staining instrument, Ventana Medical Systems). The monoclonal antibodies, pre-conjugated with biotin, were PMS-2 (AB: Ventana, Clone EPR3947), MLH-1 (AB: Ventana, Clone M1), MSH-6 (AB: Cell Marque, Clone: 44), and MSH-2 (AB: Cell Marque, Clone: 44). The IHC signal was visualized using the streptavidin–biotin complex method. All of the MMR IHC stains were independently reviewed by two pathologists (Lee C. T. and Chow N.H.), based on the revised Bethesda guidelines described previously [27 (link)]. Essentially, the signal was determined “loss” (i.e. abnormal) when their nuclear staining in tumor cells were absent in the presence of positive staining in the surrounding stromal cells. Weak but unequivocally positive signal was determined as “positive” (i.e. normal) expression.

Huang W., Ho C.L., Lee C.T., Chen W.L., Yang S.C., Chow N.H, & Chen Y.L. (2023). High concordance rate of capillary electrophoresis workflow for microsatellite instability analysis and mismatch repair (MMR) immunostaining in colorectal carcinoma. PLOS ONE, 18(4), e0284227.

+ Open protocol

+ Expand

Immunohistochemical and MSI Analysis of PDACs

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC staining of PDACs were performed using the standard streptavidin-biotin-peroxidase procedure. MMR IHC antibodies included MLH1 (Ventana, clone M1), MSH2 (Cell Marque, clone G219.1129), MSH6 (Ventana, clone 44), and PMS2 (BD Biosciences, clone A16.4). Detection systems were Bond Polymer Refine Detection (Newcastle Upon Tyne, UK) for MLH1 and PMS2, and iVIEW DAB Detection Kit (Roche Diagnostics, Indianapolis, IN) for MSH2 and MSH6. Tumors showing total absence of nuclear staining, with adjacent benign tissue showing nuclear staining, were scored as negative for expression of that protein. An experienced pathologist at MSKCC (JS) interpreted the histopathology and IHC results.
MSI analysis was performed using either the five microsatellite loci (BAT-25, BAT-26, NR-21, NR-24, and MONO-27) by PCR reaction or MSIsensor analysis. With MSI PCR testing, fluorescently labeled products were detected and sized by capillary electrophoresis. Microsatellite instability at ≥ 2 loci was defined as MSI-H, instability at a single locus was defined as MSI-L, and no instability at any of the loci tested was defined as MSS. MSIsensor interrogated the length distribution of all genomic microsatellite loci included in the MSK-IMPACT capture region across tumor and matched normal(12 (link)). MSI-H was defined as >10% of the microsatellite loci showing microsatellite instability.

Hu Z.I., Shia J., Stadler Z.K., Varghese A.M., Capanu M., Salo-Mullen E., Lowery M.A., Diaz LA J.r., Mandelker D., Yu K.H., Zervoudakis A., Kelsen D.P., Iacobuzio-Donahue C.A., Klimstra D.S., Saltz L.B., Sahin I.H, & O’Reilly E.M. (2018). Evaluating Mismatch Repair Deficiency in Pancreatic Adenocarcinoma: Challenges and Recommendations. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(6), 1326-1336.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!