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Ndei and ecori cloning sites

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NdeI and EcoRI are two commonly used restriction endonuclease enzymes that recognize and cleave specific DNA sequences. NdeI recognizes the palindromic DNA sequence 'CATATG', while EcoRI recognizes the palindromic sequence 'GAATTC'. These enzymes are frequently used in molecular biology and genetic engineering for the purpose of DNA cloning and manipulation.

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EcoRI (4 citations)

2 protocols using ndei and ecori cloning sites

1

Recombinant Expression of USP7 Constructs

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The USP7 UBL1-2 construct (residues 535–775)12 was a gift from Dr. Vivian Saridakis (York University, Toronto, Canada). This construct expresses USP7 UBL1-2 with an N-terminal 6-His tag, separated by a tobacco etch virus (TEV) protease cleavage site. The codon-optimized sequence of USP7 TRAF (residues 62–205) was chemically synthesized and subcloned into pET-28b + using NdeI and EcoRI cloning sites (Genscript).
Ashton N.W., Valles G.J., Jaiswal N., Bezsonova I, & Woodgate R. (2020). DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7. Journal of molecular biology, 433(2), 166733.
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2

Recombinant Expression of USP7 Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The USP7 UBL1-2 construct (residues 535–775)12 was a gift from Dr. Vivian Saridakis (York University, Toronto, Canada). This construct expresses USP7 UBL1-2 with an N-terminal 6-His tag, separated by a tobacco etch virus (TEV) protease cleavage site. The codon-optimized sequence of USP7 TRAF (residues 62–205) was chemically synthesized and subcloned into pET-28b + using NdeI and EcoRI cloning sites (Genscript).
Ashton N.W., Valles G.J., Jaiswal N., Bezsonova I, & Woodgate R. (2020). DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7. Journal of molecular biology, 433(2), 166733.
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