I 71 microscope

Manufactured by Olympus

The I × 71 is a high-quality microscope designed for advanced laboratory applications. It features an advanced optical system and state-of-the-art components to deliver exceptional image quality and performance. The core function of the I × 71 is to provide users with a versatile and reliable tool for detailed observation and analysis of samples.

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Lab products found in correlation

P 2000, by Narishige (1 mentions) Qcapture pro 6, by Olympus (1 mentions) Mf 900, by Narishige (1 mentions) Microtime 200 system, by PicoQuant (1 mentions)

Spelling variants of this product

1×71 microscope (17 citations) I ×71 inverted microscope (5 citations)

2 protocols using i 71 microscope

Red Blood Cell Membrane Stiffness Measurement

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A borosilicate glass micropipette was used to extract the RBC membrane shear modulus (or membrane stiffness) by micropipette aspiration technique8 (link). Pipettes were drawn from borosilicate glass tubing (Sutter Instrument Model P-2000) and cut open (Narishige MF-900) prior to mounting to the micromanipulator. The micropipette's inner diameter was approximately 1 ± 0.25 µm. A pressure drop rate of 1 Pa/s and a total pressure drop of 100 Pa were applied to aspirate and deform each cell. A total sample size of at least 20 cells per measurement was taken for individual experiments. The cell membrane was monitored by Olympus I × 71 microscope and processed by QCapture™ Pro 6.0 as described above. The maximum time span before the whole sample was replaced by a fresh sample was 1 h. From the high-resolution recordings, the leading edge of the aspired RBC membrane was tracked manually for calculating the elastic shear modulus using the Hochmuth model8 (link).

Sinha A., Chu T.T., Dao M, & Chandramohanadas R. (2015). Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress. Scientific Reports, 5, 9768.

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Fluorescence Correlation Spectroscopy of NPs

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For fluorescence correlation spectroscopy (FCS), 0.25 mg of NPs were resuspended in distilled water and incubated with 7 μg of CpG-fluorescein isothiocyanate (FITC) for 60–90 min followed centrifugation and washing at 11,000 × g for 15 min to remove excess CpG ligand. FCS measurements were done using Microtime 200 system from Picoquant GmbH (Berlin, Germany). NPs (approximately 0.25 mg) were diluted in distilled water and 30 μl of solution was dropped onto a 20 × 20 mm No. 1 coverslip (Menzel–Gläser, MA, USA). The focal height was adjusted to 20 μm above this coverslip using an Olympus i × 71 microscope and an Olympus 60 × 1.2 NA objective.

Kokate R.A., Thamake S.I., Chaudhary P., Mott B., Raut S., Vishwanatha J.K, & Jones H.P. (2015). Enhancement of anti-tumor effect of particulate vaccine delivery system by ‘Bacteriomimetic’ CpG functionalization of poly-lactic-co-glycolic acid nanoparticles. Nanomedicine (London, England), 10(6), 915-929.

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