Anti il 1beta

HCQ, LPS, nigericin, ATP, NS169, NS309, clotrimazol, IBTX, TEA and BAPTA-AM were from Sigma (St Louis). Anti-IL-1beta and anti-caspase 1 were from Santa Cruz Biotechnologies. Human IL-1beta ELISAs were from BD.

Western blot analysis was performed according to the standard procedure, using the following antibodies: oxidized low-density lipoprotein (lectine-like) receptors (ORLs) (1:1000, Novusbio), leucocyte receptor cluster member 8 (Leng8) (1:2000 Novusbio), vomoronasal receptor-1 (Vnr1) (1:2000 Abcam), tetratricopeptide repeat and ankyrin repeat containing1 (Trank1) (1:1000 Novusbio), matrix metalloproteinase 9 (MMP9) (1:1000 Abcam), matrix metalloproteinase 2 (MMP2) (1:1000 Abcam), occludin 1 (1:1000, Abcam), plasmalemmal vesicle associated protein-1 (PV-1) (1:2000 Abcam), IL-6 (1:2000 Santa Cruz, CA, USA), anti-TNF (1:600 Santa Cruz, CA, USA), anti-IL-1 beta (1:4000 Santa Cruz, CA, USA), anti-IL-10 (1:2000 Peprotec, London, UK), and beta-actin (1:4000, Sigma-Aldrich). Fifty micrograms of proteins per lane were diluted in loading buffer and denatured at 70 °C for 10 min. Optical densities of the bands were measured using ImageJ software (http://rsb.info.nih.gov/ij/) and normalized against beta-actin.