miRNA quantitative polymerase chain reactions (qPCR) were performed using the TaqMan MicroRNA reverse transcription kit (Life Technologies), Taqman MicroRNA qPCR assays (Life Technologies) and TaqMan Universal PCR Master Mix, as previously described [35] (link). qPCR reactions were performed according to the manufacturers’ suggested conditions. Sno202 was used as a housekeeping control RNA in the experiments. Relative expression was calculated using the 2−ΔΔCt method.
Taqman microrna qpcr assays
Manufactured by Thermo Fisher Scientific
Taqman MicroRNA qPCR assays are a set of laboratory products designed for the quantitative analysis of microRNA (miRNA) expression levels using real-time PCR technology. These assays provide a standardized and sensitive method for the detection and quantification of miRNA molecules in various sample types.
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Lab products found in correlation
2 protocols using taqman microrna qpcr assays
1
Quantitative miRNA Expression Analysis
2
miRNA qPCR Reaction Protocol
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MiRNA qPCR reactions were performed using the TaqMan MicroRNA reverse transcription kit (Life Technologies), Taqman MicroRNA qPCR assays (Life Technologies) and TaqMan Universal PCR Master Mix, as previously described [25 (link), 26 (link)]. Briefly, cDNA was reverse transcribed from total RNA using specific miRNA primers according to the suggested reaction conditions on a BioRad T100 thermal cycler. Quantitative polymerase chain reaction (qPCR) was performed using a ViiA 7 Real Time PCR System (Applied Biosystems, Carlsbad, CA). Sno202 was used as a housekeeping control RNA. Relative expression was calculated using the 2−ΔΔCtmethod.
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