Bay 60 7550

Aβ1–42 was obtained from rPeptide. Bay 60-7550 (CAS: 439083-90-6) was obtained from Sigma-Aldrich. The mice were treated with different doses of Bay 60-7550 (0.5, 1.0, 3.0 mg/kg/day, i.p.) or vehicle for 14 days after microinjection of Aβ1–42. PKG inhibitor KT5823 (Cayman Chemical, United States) and PKA inhibitor H89 (Sigma-Aldrich, United States) were microinjected bilaterally into the cerebroventricular, 30 min prior to treatment with Bay 60-7550. Antibodies against CRF, GR, p-CREB, CREB, and BDNF were obtained from Abcam (Cambridge, MA, United States). All secondary antibodies were obtained from Beyotime Biotechnology (Haimen, China).

The Gateway LR Clonase Enzyme Kit was from Thermo Fischer Scientific (Dreieich, Germany). The cilostamide was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). BAY 60-7550, and all other chemicals were from Sigma-Aldrich (Deisenhofen, Germany).