Am2200 analog stimulus isolator

To stimulate and record from the animal, a data acquisition box (DAQ) is coupled to an analog stimulator and an extracellular amplifier. Analog output waveforms from the DAQ control the stimulator, which in turn stimulates the nerve in the animal, and electromyographic (EMG) waveforms are received by the DAQ’s input after amplification (Fig. 1C). The data acquisition box used in this study was an NI-USB-6361 (National Instruments, TX, USA) connected to a computer running Windows 10. Stimuli were provided by an AM2200 Analog Stimulus Isolator (A–M Systems, WA, USA), and the extracellular amplifier used to gather EMG signals was an AM1700 Differential AC Amplifier (A–M Systems, WA, USA). The SIGNAL IN port of the stimulator was connected to the Analog Output of the DAQ. The polarity of the stimulator was set to bipolar, and the range set to 1 mA/V for all measurements. Stimulating electrodes were connected to the Output ports of the AM2200 stimulator. EMG electrodes were connected to the AM1700 amplifier (Fig. 1C). The recording gain was typically set to 1000. The notch filter provided was turned on, and the animal ground was supplied by the amplifier.